TOXICOLOGICAL SCREENING METHOD BY HPLC-DAD WITH LOCAL SPECTRAL LIBRARY AND "HOME-MADE" INTERNAL STANDARD

Authors

  • Khaled Sobhi Central Hospital of the Army
  • Souhila Mélaïne
  • Abderrahmane Abdaoui
  • Mohamed Mammar

DOI:

https://doi.org/10.4314/jfas.1180

Keywords:

HPLC-DAD, Liquid-Liquid extraction, drug poisoning, systematic toxicological analysis

Abstract

A liquid-liquid extraction technique and HPLC-DAD method using a C18 column, 5μm (150x4.6mm i.d.) were developed for a toxicological drug screening. A home-made “N-acetylparoxetine” is used as internal standard. A local UV spectra library of over 130 drugs and some of their metabolites was created. This library covers the main therapeutic classes involved in intoxication cases. The developed procedure is successfully applied to different biological samples (serum, urine, gastric fluid and post mortem whole blood) and allows rapid and simultaneous detection and identification of a large number of substances and their semi-quantification in serum.

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References

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Published

2022-04-30

How to Cite

SOBHI, K.; MÉLAÏNE, S.; ABDAOUI, A.; MAMMAR, M. TOXICOLOGICAL SCREENING METHOD BY HPLC-DAD WITH LOCAL SPECTRAL LIBRARY AND "HOME-MADE" INTERNAL STANDARD. Journal of Fundamental and Applied Sciences, [S. l.], v. 14, n. 2, p. 351–367, 2022. DOI: 10.4314/jfas.1180. Disponível em: https://jfas.info/index.php/JFAS/article/view/1180. Acesso em: 13 jul. 2024.

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