EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE SPONGE AXINELLA DONNANI AND ITS POTENTIAL CYTOTOXIC ACTIVITY

Ratheesh Sadanandan; Arun A. Rauf

doi:10.4314/jfas.1195

Authors

Ratheesh Sadanandan Department of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India
Arun A. Rauf Department of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India

DOI:

https://doi.org/10.4314/jfas.1195

Keywords:

Axinella donnani, Lectin, MCF-7, A431, HeLa, MTT assay

Abstract

Marine sponges are oldest metazoans which were found to have structural and biologically diverse lectins. A lectin isolated from marine sponge Axinella donnani (ADL) were evaluated for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Guanidine hydrochloride does not show any activity. Finally, cytotoxicity of ADL at different concentrations (0.25-500 mg/mL) was tested against MCF-7, A431 and HeLa cell lines by MTT assay. ADL exhibited potential cytotoxicity against these cell lines after 24 hours in a dose dependent manner. Thus, ADL could be a potent cytotoxic agent in clinical applications.

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References

Rudi A, Yosief T, Schleyer M & Kasbman Y. Several new isoprenoids from two marine sponges of the family Axinellidae. Tetrahedron, 1999, 55: 5555–5566.

Carletti, I., Long, C., Funel, C., Amade, P.: Yardanone A and B, New Cytotoxic Triterpenes From the Indian Ocean Sponge Axinella cf. bidderi. J. Nat. Prod., 66, 25 (2003).

Fernandez, J.J., Souto, M.L., Norte, M.: Marine Polyether Triterpenes. Nat. Prod. Rep., 2000, 17, 235.

Biju Kumar, A. 2012. ‘Kerala Theerathe Kadal Jeevikal’ (Marine Animals of Kerala coast- A Field Guide). Kerala State Biodiversity Board, Thiruvananthapuram, Kerala, 304 pp. (In Malayalam).

Van Soest, R. (2014). Axinella donnani (Bowerbank, 1873). In: Van Soest, R.W.M; Boury-Esnault, N.; Hooper, J.N.A.; Rützler, K.; de Voogd, N.J.; Alvarez de Glasby, B.; Hajdu, E.; Pisera, A.B.; Manconi, R.; Schoenberg, C.; Janussen, D.; Tabachnick, K.R., Klautau, M.; Picton, B.; Kelly, M.; Vacelet, J.; Dohrmann, M.; Díaz, M.-C.; Cárdenas, P. (2014) World Porifera database. Accessed through: World Register of Marine Species at http://www.marinespecies.org/aphia.php?p=taxdetails&id=165477 on 2014-12-10.

Selvin, J.; Lipton, A.P. Biopotentials of Secondary Metabolites Isolated from Marine Sponges. Hydrobiologia; 2004, 513, 231–238.

Vimala, Antonydhason & Innocent, B. (2015). Phylogenetic Investigations on the Endosymbiotic Bacteria of Axinella donnani. Journal of Microbial & Biochemical Technology. 07. https://doi.org/10.4172/1948-5948.1000232

Sharon, Nathan. Lectins: Carbohydrate-specific Reagents and Biological Recognition Molecules. The Journal of biological chemistry. 2008, 282. 2753-64. https://doi.org/10.1074/jbc.X600004200

Gomes Filho SM, Cardoso JD, Anaya K, et al. Marine sponge lectins: actual status on properties and biological activities. Molecules. 2014; 20(1) :348-357. doi: https://doi.org/10.3390/molecules20010348

Cheung, R. C., Wong, J. H., Pan, W., Chan, Y. S., Yin, C., Dan, X., & Ng, T. B. Marine lectins and their medicinal applications. Applied microbiology and biotechnology, , 2015, 99(9), 3755–3773. https://doi.org/10.1007/s00253-015-6518-0

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63.

Zegheb N, Boubekri C, Lanez T, Lanez E, Tüylü Küçükkılınç T, Öz E, Khennoufa A, Khamouli S, Belaidi S. In vitro and in silico determination of some N-ferrocenylmethylaniline derivatives as anti-proliferative agents against MCF-7 human breast cancer cell lines. Anti-Cancer Agents in Medicinal Chemistry, 2022, 22(7), 1426-1437.

https://doi.org/10.2174/1871520621666210624141712

Kaur N, Singh J, Kamboj SS. Affinity purification and characterization of seed lectin from Crotalaria medicaginea. Indian J. Biochem. Biophys.2002; 39: 49-54.

Ratheesh Sadanandan and Arun. A. Rauf, Isolation, Purification and Characterisation of a D-galactose and N-acetyl-D-galactosamine Specific Lectin from Marine Sponge Fasciospongia Cavernosa, Protein & Peptide Letters, 2018, 25, 1-7.

Singh AP and Saxsena KD, Chem Sci Trans.2013; 2(1), 258-262.

Bains JS, Dhuna V, Singh J, Kamboj SS, Nijjar KK, Agrewala JN. Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines. International Immunopharmacology. 2005; 5: 1470–1478.

Yalçın, F. N. Biological Activities of the Marine Sponge Axinella Hacettepe University Journal of the Faculty of Pharmacy. 2007 .27 ;1: pp. 47-60.

Dresch RR, Lerner CB, Mothes B, Trindade VMT, Henriques AT, Vozári-Hampe MM. Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata. Comp. Biochem. Physiol. Biochem. Mol. Biol. 2012; 161: 365–370.

Pajic I, Kljajic Z, Dogovic N, Sladic D, Juranic Z, Gasic MJ. A novel lectin from the sponge Haliclona cratera: Isolation, characterization and biological activity. Comp. Biochem. Physiol. Toxicol. Pharmacol.2002; 132: 213–221.

Rabelo L, Monteiro N, Serquiz R, Santos P, Oliveira R, Oliveira A, Rocha H, Morais AH, Uchoa A, Santos E. A Lactose-Binding Lectin from the Marine Sponge Cinachyrella Apion (Cal) Induces Cell Death in Human Cervical Adenocarcinoma Cells. Marine Drugs. 2012; 10(4):727-743. https://doi.org/10.3390/md10040727

Queiroz AF, Silva RA, Moura RM, Dreyfuss JL, Paredes-Gamero EJ, Souza AC, Tersariol IL, Santos EA, Nader HB, Justo GZ, de Sales MP. Growth inhibitory activity of a novel lectin from Cliona varians against K562 human erythroleukemia cells. Cancer Chemother Pharmacol. 2009;63(6):1023–1033.

Nascimento-Neto, L. G., Cabral, M. G., Carneiro, R. F., Silva, Z., Arruda, F., Nagano, C. S., Fernandes, A. R., Sampaio, A. H., Teixeira, E. H., & Videira, P. A. (2018). Halilectin-3, a Lectin from the Marine Sponge Haliclona caerulea, Induces Apoptosis and Autophagy in Human Breast Cancer MCF7 Cells Through Caspase-9 Pathway and LC3-II Protein Expression. Anti-cancer agents in medicinal chemistry, 18(4), 521–528.

https://doi.org/10.2174/1871520617666171114094847