• Yarma Ahmed Adamu Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, Gombe State, Nigeria
  • Mustapha S.H Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, Gombe State, Nigeria
  • Lawal Garba Department of Microbiology, Gombe State University
  • Muhammed Tukur Adamu Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, Gombe State, Nigeria



Bryophyllum pinnatum, Sensitivity test, Styaphlococous aureus, Escherichica coli


Pathogenic microorganisms cause several infections of great public concern. These pathogens from many studies were found to be resistant to many antibiotics. Thus there is a need for new antimicrobial agents of plant origin to overcome the problem of resistance. The aim of this study was to screen the antibacterial activity of the aqueous leave extract of Bryophyllum pinnatun. The aqueous leave extract of the B. pinnatun prepared by the percolation method was investigated for phytochemical constituents and activity against Staphylococcus aureus and Escherichia coli using the agar well diffusion method. The phytochemicals detected in the extract include saponins, tannins, alkaloids and flavonoids. The extract was found to be active on both organisms with a zone of inhibition ranging from 8-19mm for S. aureus and 7-16mm for E. coli. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were found to be higher at 50mg/ml and 100mg/ml, respectively. This study shows that the leaf extract of B. pinnatum have antibacterial properties, and is effective on both Gram-positive and Gram-negative bacteria. Therefore, this result supports the traditional use of the B. pinnatum leaves in infection treatment.


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Aslam, B., Wang, W., Arshad, M. I., Khurshid, M., Muzammil, S., Rasool, M. H., Baloch, Z., Antibiotic resistance: a rundown of a global crisis. Infection and drug resistance, 11, 2018, 1645.

Rossolini, G. M., Arena, F., Pecile, P., Pollini, S., Update on the antibiotic resistance crisis. Current opinion in pharmacology, 18, 2014, 56-60.

Owusu, E., Ahorlu, M. M., Afutu, E., Akumwena, A., Asare, G. A., Antimicrobial activity of selected medicinal plants from a sub-Saharan African country against bacterial pathogens from post-operative wound infections. Medical Sciences, 9(2), 2021, 23.

Manandhar, S., Luitel, S., & Dahal, R. K. Journal of tropical medicine, 2019, 2019. 1895340. doi:

World Health Organization. The world health report 2002: reducing risks, promoting healthy life. World Health Organization. Traditional Medicine Strategy, Geneva, 2002.

Nagaratna, A., Hegde, P. L., A comprehensive review on Parnabeeja [Bryophyllum pinnatum (Lam.) Oken]. J med plants stud, 3(5), 2015, 166-71.

Ghasi, S., Egwuibe, C., Achukwu, P. U., Onyeanusi, J. C., Assessment of the medical benefit in the folkloric use of Bryophyllum Pinnatum leaf among the Igbos of Nigeria for the treatment of hypertension. African journal of pharmacy and pharmacology, 5(1), 2011, 83-92.

Obioma, A., Chikanka, A. T., & Dumo, I. Annals of Clinical and Laboratory Research, 5(3), 2017, 1-6. doi: 10.21767/2386-5180.1000185

Gill, L. S., Ethnomedical uses of plants in Nigeria. Published by University of UniBenin Press, University of Benin. Benin City, Edo State, Nigeria, 46, 1992, 143

Edeoga, H. O., Okwu, D. E., Mbaebie, B. O., Phytochemical constituents of some Nigerian medicinal plants. African journal of biotechnology, 4(7), 2005, 685-688.

Iwu, M.M., In vitro Antiviral activities of Bryophyllum pinnatum, 1993

Truong, D. H., Nguyen, D. H., Ta, N. T. A., Bui, A. V., Do, T. H., Nguyen, H. C., Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. Journal of food quality, 2019, 2019.

Aibinu, I., Adenipekun, T., Adelowotan, T., Ogunsanya, T., Odugbemi, T., Evaluation of the antimicrobial properties of different parts of Citrus aurantifolia (lime fruit) as used locally. African Journal of Traditional, Complementary, and Alternative Medicines, 4(2), 2007, 185.

Mudi, S. Y., Ibrahim, H., Activity of Bryophyllum pinnatum S. Kurz extracts on respiratory tract pathogenic bacteria. Bayero Journal of Pure and Applied Sciences, 2008, 39-42.

Ojewole, J. A., Antinociceptive, anti-inflammatory and antidiabetic effects of Bryophyllum pinnatum (Crassulaceae) leaf aqueous extract. Journal of ethnopharmacology, 99(1), 2005, 13-19.

Ofokansi, K. C., Esimone, C. O., Anele, C. R., Evaluation of the in vitro combinedantibacterial effect of the leaf extracts of Bryophyllum pinnatum (Fam: Crassulaceae) and Ocimum gratissimum (Fam: Labiatae). Plant Products Research Journal, 9, 2005, 23-27.

Okwu, D. E., Josiah, C., Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology, 5(4), 2006, 357-361.

Lanez, E., Zegheb, N., Lanez, T. Turkish Computational and Theoretical Chemistry, 7(3), 2023, 10-16. Doi:

Akacha, L., Dikko, J., Khan, M., Anyam, J., Igoli, J., Phytochemical screening and antimicrobial activity of Bryophyllum pinnatum extracts. British Biotechnology Journal, 16(2) 2016, 1-8.

Yusha’u M., Garba L., Shamsuddeen, U., In vitro inhibitory activity of garlic and ginger extracts on some respiratory tract isolates of gram-negative organisms. International Journal of Biomedical and Health Sciences, 4(2), 2008.

Garba, L., Abdulfatah, A., Adamu, M. T., Ismail, H., Yusuf, I., Dahiru, A. S., Evaluation of Antibacterial Potency of Endophytic Fungi Isolated from Mentha piperita. International Journal of Innovative Approaches in Science Research, 5 (4), 2021, 251-262.

Audu, S. A., Mohammed, I., Kaita, H. A., Phytochemical screening of the leaves of Lophira lanceolata (Ochanaceae). Life Science Journal, 4(4), 2007, 75-79.

Cheesbrough, M., District Laboratory Practice in Tropical Africa. University of Cambridge 2nd Edition Update Part, 2, 2006, 63-70.

Garba, L., Lawan, H. S., Puma, H. U., Abdullahi, M. M., Yusuf, I., Mukhtar, M. D., Phytochemical Screening and in vitro Bacteriostatic Effects of Syzigium aromaticum (Clove) Extracts on Clinical Bacterial Isolates. Journal of Biochemistry, Microbiology and Biotechnology, 7(1), 2019, 5-9.

Garba, L., Muhammad, M. B., Adamu, M. T., Isa, S., Abdullahi, M. M., Yarma, A. A., Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria. Journal of Fundamental and Applied Sciences, 13(1), 2021, 137-150.

Adetitun, D. O., Araoye, H. K., Akinyanju, J. A., Anibijuwon, I. I., Antimicrobial Effect of the Leaf Extracts of Moringa oleifera on Some Selected Clinical Bacterial Isolates. Agrosearch, 13(1), 2013, 95-114.

Pacheco‐Ordaz, R., Wall‐Medrano, A., Goñi, M. G., Ramos‐Clamont‐Montfort, G., Ayala‐Zavala, J. F., González‐Aguilar, G. A., Effect of phenolic compounds on the growth of selected probiotic and pathogenic bacteria. Letters in applied microbiology, 66(1), 2018, 25-31.




How to Cite

ADAMU, Y. A.; S.H, M. .; GARBA, L.; ADAMU , M. T. . ANTIBACTERIAL ACTIVITY OF BRYOPHYLLUM PINNATUM LEAVES EXTRACT AGAINST STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI. Journal of Fundamental and Applied Sciences, [S. l.], v. 15, n. 3, p. 230–240, 2023. DOI: 10.4314/jfas.1322. Disponível em: Acesso em: 28 nov. 2023.