ANTIMITOTIC AND ANTIPROLIFERATIVE ACTIVITIES OF CRUDE FUNGAL EXTRACTS OF ENDOPHYTIC FOLIAR FUNGI OF PEGANUM HARMALA L. FROM DAYATE AIAT (LAGHOUAT, ALGERIA)
DOI:
https://doi.org/10.4314/jfas.v11i2.3Keywords:
Peganum harmala L., foliar endophytic fungi, fungal extracts, antimitotic activity, antiproliferative activity, Laghouat (Algeria).Abstract
Medicinal plants are an inexhaustible source of bioactive molecules. They can be produced by the various organs of the plant or by mutual endophytic fungi present within the latter. It is with this in mind that we were interested in a spontaneous plant in the arid zones of Algeria (Laghouat): Peganum harmala L. Our interest in this work focused on the demonstration of the antimitotic and antiproliferative activities of the extracts. Cranial fungi of the genera Cladosporium, Alternaria, Aspergillus and Penicillium: endophytic foliar fungi of Peganum harmala L. and their different combinations. The extraction of crude fungal secondary metabolites was carried out using ethyl acetate. The results confirm their action in inhibiting mitosis, as well as the combinations of extracts made, thus producing chromosomal and cellular abnormalities. Tests on Saccharomyces cerevisiae also indicate the presence of antiproliferative activity of the various fungal extracts, thus reflecting a cytotoxic effect.
Key words: Peganum harmala L., foliar endophytic fungi, fungal extracts, antimitotic activity, antiproliferative activity, Laghouat (Algeria).
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References
[1] Zhang HW, Song YC and Tan RX. Biology and chemistry of endophytes. Nat Prod Rep., 2006, 23: 753–771.
[2] Schulz B, Boyle C, Draeger S, Rommert AK and Krohn K. Endophytic fungi:a source of novel biologically active secondary metabolites. Mycol Res., 2002, 106: 996–1004.
[3] Kaul S, Gupta S, Ahmed M and K. Dhar M. Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites. Phytochem Rev., 2012, 11(4): 487–505.
[4] Bourogaa E, Jarraya RM, Damak M and Elfeki A. Hepatoprotective activity of Peganum harmala against ethanol-induced liver damages in rats. Arch Physiol Biochem., 2015, 121(2): 62-7.
[5] Bukhari B, Choi JH, Jeon CW, Park HW, Kim WH, Khan MA and Leet SH. Phytochemical studies of the alkaloids from Peganum harmala. Appl. Chem., 2008, 12: 101–104.
[6] Jinous A and Fereshteh R. Chemistry, pharmacology and medicinal properties of Peganum harmala L. Afr J Pharm Pharmacol., 2012, 6: 1573–1580.
[7] Hilal SH, Haggag MY, Soliman F and El-Kashoury S. Alkaloids of Peganum harmala L. Egypt Pharm Sci., 1978, 19: 393–399.
[8] Boeira JM, Da Silva J, Erdtmann B, and Henriques JAP. Genotoxic Effects of the Alkaloids Harman and Harmine Assessed by Comet Assay and Chromosome Aberration Test in Mammalian Cells in vitro. Pharmacology & Toxicology, 2001, 89: 287.
[9] Go¨Ckler N, Jofre G, Papadopoulos C, Soppa U, Tejedorand FJ and Becker W. Harmine specifically inhibits protein kinase DYRK1A and interferes with neurite formation. FEBS Journal, 2009, 276: 6324.
[10] Adhami HR, Farsam H and Krenn L. Screening of Medicinal Plants from Iranian Traditional Medicine for Acetylcholinesterase Inhibition. Phytotherapy Research, 2011,25: 1148.
[11] Schulz B, Wanke S, Draeger S and Aust HJ. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol. Res., 1993, 97: 1447-1450.
[12] Ellis MB. Dematiaceus hyphomycetes. Commonwealth Mycological Institute, Kew, 1971.
[13] Watanabe T. Soil and seed fungi. Morphologies of cultured fungi and key to species. CRC press, London, 2002.
[14] Domsch KH, Gams W and Anderson TH. Compendium of soil fungi. IHW-Verlag, Eching, 2007.
[15] Samson RA, Houbraken J, Thrane U, Frisvad JC and Andersen B. Food and indoor fungi. CBS-KNAW Fungal Biodiversity Centre, Utrecht, 2010.
[16] Xiaoling C, Xiaoli L, Shining Z, Junping G, Shuiping W, Xiaoming L, Zhigang S and Yongcheng L. Cytotoxic and topoisomerase I inhibitory activities from extracts of endophytic fungi isolated from mangrove plants in Zhuhai, China. Journal of Ecology and The Natural Environment, 2010, 2: 017-024.
[17] Barik BP, Tayung K, Jagadev PN and Dutta SK. Phylogenetic placement of an endophytic fungus Fusarium oxysporum isolated from Acorus calamus rhizomes with antimicrobial activity. European Journal of Biological Sciences, 2010, 2: 8-16.
[18] Mohanta J, Tayung K and Mohapatra U. Antimicrobial potentials of endophytic fungi inhabiting three Ethnomedicinal plants of Similipal Biosphere Reserve, India. The Internet Journal of Microbiology, 2008, 5(2):122-132.
[19] Madki MA, Manzoor AS, Powar PV and Patil K S. Isolation and Biological Activity of Endophytic Fungi fromWithania Somnifera. International Journal of Pharmaceutical Sciences , 2010, 2: 848-858.
[20] Badji B, Riba A, Mathieu F, Lebrihi A and Sabaou N. Activité antifongique d'une souche d'Actinomadura d'origine saharienne sur divers champignons pathogènes et toxinogènes. Journal de Mycologie Medicale, 2005, 4 (15): 211-219.
[21] Gogoi DK, Mazumder S, Saikia R and Bora TC. Impact of submerged culture conditions on growth and bioactive metabolite produced by endophyte Hypocrea spp. NSF-08 isolated from Dillenia indica Linn. in North-East India. Journal of Medical Mycology, 2008,1(18): 1–9.
[22] Qin JC, Zhang YM, Gao JM, Bai MS, Yang SX, Laatsch H and Zhang AL. Bioactive metabolites produced by Chaetomium globosum, an endophytic fungus isolated from Ginkgo biloba. Bioorganic & Medicinal Chemistry Letters, 2009, 6(19):1572-1574.
[23] Oliveira Silva MR, Sena Xisto K and Buarque Gusumao N. Secondary metabolites produced by endophytic fungus Paecilomyces variotii Bainier with antimicrobial activity against Enterococcus faecalis. In: Mendez-Vilas A.(eds). Current Research Topics in Applied Microbiology: Proceedings of the II international Conference on Environmental, Indutrial and Applied Microbiology (Biomicroworld2007), 2009, 519-520.
[24] Shweta S, Khadabadi S and Ganesh TG. In vitro antimitotic, antiproliferative, DNA fragmentation and anticancer activity of chloroform and ethanol extract of Revia hypocrarerformis. Asian Pacific J Trop Dis., 2012, S503-06.
[25] Subhadradevi V, Khairunissa K, Asokkumar K, Umamaheswari M, Sivashanmugam A and Jagannath P. Induction of apoptosis and cytotoxic activities of Apium graveolens Linn. using in vitro models. Middle-East J Sci Res., 2011, 9: 90-94.
[26] Sehgal R, Roy S and Kumar VL. Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allum cepa root model. J Biocell., 2006, 30: 9-13.
[27] Channabasava and Govindappa M. First report of anticancer agent, lapachol producing endophyte, Aspergillus niger of Tabebuia argentea and its in vitro cytotoxicity assays. Bangladesh J Pharmacol., 2014, 9: 129-139.
[28] Brase S, Encinas A, Keck J and Nising CF. Chemistry and biology of mycotoxins and related fungal metabolites. Chem. Rev., 2009, 109: 3903–3990.
[29] Tsuge T, Harimoto Y, Akimitsu K, Ohtani K, Kodama M, Akagi Y, Egusa M, Yamamoto M and Otani H. Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata. FEMS Microbiol. Rev., 2013, 37: 44–66.
[30] Stierle A, Strobel GA and Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science, 1993, 260: 214-16.
[31] Li JY, Stroble GA, Sidhu R, Hess WM and Ford EJ. Endophytic Taxol producing fungi from bald cypress, Taxodium distichum. Microbiology, 1996, 142: 2223-2226.
[32] Visalakchi S and Muthumary J. Taxol (anticancer drug) producing endophytic fungi: An overview. Int J Pharma Bio Sci. 2010, 1: 1-9.
[33] Zhao J, Shan T, Mou Y and Zhou L. Plant-Derived Bioactive Compounds Produced by Endophytic Fungi. Mini-Reviews in Medicinal Chemistry, 2011, 11: 159-168.
[34] Schiff PB, Fant J and Horwitz SB. Promotion of microtubule assembly in vitro by taxol. Nature, 1979, 277: 665-667.
[35] Wang LG, Liu XM, Kreis W and Budman DR. The effect of antimicrotubule agents on signal transduction pathways of apoptosis: a review. Cancer Chemother. Pharm., 1999, 44: 355-361.
[36] Sadananda TS, Nirupama R, Chaithra K, Govindappa M, Chandrappa CP and Vinay Raghavendra B. Antimicrobial and antioxidant activities of endophytes from Tabebuia argentea and identification of anticancer agent (lapachol). J Med Plant Res., 2011,5: 3643-52.
[37] Yu SF, Chen TM and Chen YH. Apoptosis and necrosis are involved in the toxicity of Sauropus androgynus in an in vitro study. J Formos Med Assoc., 2007, 106: 537-47.
[38] Türk M, Kaya B, Menemen Y and Oğuztüzün S. Apoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla L. species on HeLa cells in vitro. J Med Plants Res., 2011, 5: 4566-71.
[39] Caruso M, Colombo AL, Fedeli L, Pavesi A, Quaroni S, Saracchi M and Ventrella G. Isolation of endophytic fungi and actinomycetes taxane producers. Ann. Microbiol., 2000, 50: 3–14.
[40] Guo B, Li H and Zhang L. Isolation of a fungus producting vinblastine. J. Yunnan Univ. Nat. Sci., 1998, 20: 214–215.
[41] Ya-Tuan M, Li-Rui Q, Wen-Quan S, An-Ling Z and Jin-Ming G. Metabolites produced by an endophyte Alternaria alternata isolated from Maytenus hookeri.Chem.Nat.Compd., 2010, 46 (3).
[42] Zaferanloo B, Quang T D, Daumoo S, Ghorbani MM, Mahon PJ and Palombo EA. Optimization of protease production by endophytic fungus, Alternaria alternata isolated from an Australian native plant. World J. Microbiol. Biotechnol., 2014, 30(6): 1755-1762.
[43] Eyberger AL, Dondapati R and Porter JR. Endophyte fungal isolates from Podophyllum peltatum produce podophyllotoxin. J. Nat. Prod., 2006, 69: 1121-1124.
[44] Cao L, Huang J and Li J. Fermentation conditions of Sinopodophyllum hexandrum endophytic fungus on production of podophyllotoxin. Food Fermentation Ind., 2007, 33: 28-32.
[45] Kour A, Shawl AS, Rehman S, Sultan P, Qazi PH, Suden P, Khajuria RK and Verma V. Isolation and identification of an endophytic strain of Fusarium oxysporum producing podophyllotoxin from Juniperus recurva. World J. Microbiol. Biotechnol., 2008, 24: 1115-1121.
[2] Schulz B, Boyle C, Draeger S, Rommert AK and Krohn K. Endophytic fungi:a source of novel biologically active secondary metabolites. Mycol Res., 2002, 106: 996–1004.
[3] Kaul S, Gupta S, Ahmed M and K. Dhar M. Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites. Phytochem Rev., 2012, 11(4): 487–505.
[4] Bourogaa E, Jarraya RM, Damak M and Elfeki A. Hepatoprotective activity of Peganum harmala against ethanol-induced liver damages in rats. Arch Physiol Biochem., 2015, 121(2): 62-7.
[5] Bukhari B, Choi JH, Jeon CW, Park HW, Kim WH, Khan MA and Leet SH. Phytochemical studies of the alkaloids from Peganum harmala. Appl. Chem., 2008, 12: 101–104.
[6] Jinous A and Fereshteh R. Chemistry, pharmacology and medicinal properties of Peganum harmala L. Afr J Pharm Pharmacol., 2012, 6: 1573–1580.
[7] Hilal SH, Haggag MY, Soliman F and El-Kashoury S. Alkaloids of Peganum harmala L. Egypt Pharm Sci., 1978, 19: 393–399.
[8] Boeira JM, Da Silva J, Erdtmann B, and Henriques JAP. Genotoxic Effects of the Alkaloids Harman and Harmine Assessed by Comet Assay and Chromosome Aberration Test in Mammalian Cells in vitro. Pharmacology & Toxicology, 2001, 89: 287.
[9] Go¨Ckler N, Jofre G, Papadopoulos C, Soppa U, Tejedorand FJ and Becker W. Harmine specifically inhibits protein kinase DYRK1A and interferes with neurite formation. FEBS Journal, 2009, 276: 6324.
[10] Adhami HR, Farsam H and Krenn L. Screening of Medicinal Plants from Iranian Traditional Medicine for Acetylcholinesterase Inhibition. Phytotherapy Research, 2011,25: 1148.
[11] Schulz B, Wanke S, Draeger S and Aust HJ. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol. Res., 1993, 97: 1447-1450.
[12] Ellis MB. Dematiaceus hyphomycetes. Commonwealth Mycological Institute, Kew, 1971.
[13] Watanabe T. Soil and seed fungi. Morphologies of cultured fungi and key to species. CRC press, London, 2002.
[14] Domsch KH, Gams W and Anderson TH. Compendium of soil fungi. IHW-Verlag, Eching, 2007.
[15] Samson RA, Houbraken J, Thrane U, Frisvad JC and Andersen B. Food and indoor fungi. CBS-KNAW Fungal Biodiversity Centre, Utrecht, 2010.
[16] Xiaoling C, Xiaoli L, Shining Z, Junping G, Shuiping W, Xiaoming L, Zhigang S and Yongcheng L. Cytotoxic and topoisomerase I inhibitory activities from extracts of endophytic fungi isolated from mangrove plants in Zhuhai, China. Journal of Ecology and The Natural Environment, 2010, 2: 017-024.
[17] Barik BP, Tayung K, Jagadev PN and Dutta SK. Phylogenetic placement of an endophytic fungus Fusarium oxysporum isolated from Acorus calamus rhizomes with antimicrobial activity. European Journal of Biological Sciences, 2010, 2: 8-16.
[18] Mohanta J, Tayung K and Mohapatra U. Antimicrobial potentials of endophytic fungi inhabiting three Ethnomedicinal plants of Similipal Biosphere Reserve, India. The Internet Journal of Microbiology, 2008, 5(2):122-132.
[19] Madki MA, Manzoor AS, Powar PV and Patil K S. Isolation and Biological Activity of Endophytic Fungi fromWithania Somnifera. International Journal of Pharmaceutical Sciences , 2010, 2: 848-858.
[20] Badji B, Riba A, Mathieu F, Lebrihi A and Sabaou N. Activité antifongique d'une souche d'Actinomadura d'origine saharienne sur divers champignons pathogènes et toxinogènes. Journal de Mycologie Medicale, 2005, 4 (15): 211-219.
[21] Gogoi DK, Mazumder S, Saikia R and Bora TC. Impact of submerged culture conditions on growth and bioactive metabolite produced by endophyte Hypocrea spp. NSF-08 isolated from Dillenia indica Linn. in North-East India. Journal of Medical Mycology, 2008,1(18): 1–9.
[22] Qin JC, Zhang YM, Gao JM, Bai MS, Yang SX, Laatsch H and Zhang AL. Bioactive metabolites produced by Chaetomium globosum, an endophytic fungus isolated from Ginkgo biloba. Bioorganic & Medicinal Chemistry Letters, 2009, 6(19):1572-1574.
[23] Oliveira Silva MR, Sena Xisto K and Buarque Gusumao N. Secondary metabolites produced by endophytic fungus Paecilomyces variotii Bainier with antimicrobial activity against Enterococcus faecalis. In: Mendez-Vilas A.(eds). Current Research Topics in Applied Microbiology: Proceedings of the II international Conference on Environmental, Indutrial and Applied Microbiology (Biomicroworld2007), 2009, 519-520.
[24] Shweta S, Khadabadi S and Ganesh TG. In vitro antimitotic, antiproliferative, DNA fragmentation and anticancer activity of chloroform and ethanol extract of Revia hypocrarerformis. Asian Pacific J Trop Dis., 2012, S503-06.
[25] Subhadradevi V, Khairunissa K, Asokkumar K, Umamaheswari M, Sivashanmugam A and Jagannath P. Induction of apoptosis and cytotoxic activities of Apium graveolens Linn. using in vitro models. Middle-East J Sci Res., 2011, 9: 90-94.
[26] Sehgal R, Roy S and Kumar VL. Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allum cepa root model. J Biocell., 2006, 30: 9-13.
[27] Channabasava and Govindappa M. First report of anticancer agent, lapachol producing endophyte, Aspergillus niger of Tabebuia argentea and its in vitro cytotoxicity assays. Bangladesh J Pharmacol., 2014, 9: 129-139.
[28] Brase S, Encinas A, Keck J and Nising CF. Chemistry and biology of mycotoxins and related fungal metabolites. Chem. Rev., 2009, 109: 3903–3990.
[29] Tsuge T, Harimoto Y, Akimitsu K, Ohtani K, Kodama M, Akagi Y, Egusa M, Yamamoto M and Otani H. Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata. FEMS Microbiol. Rev., 2013, 37: 44–66.
[30] Stierle A, Strobel GA and Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science, 1993, 260: 214-16.
[31] Li JY, Stroble GA, Sidhu R, Hess WM and Ford EJ. Endophytic Taxol producing fungi from bald cypress, Taxodium distichum. Microbiology, 1996, 142: 2223-2226.
[32] Visalakchi S and Muthumary J. Taxol (anticancer drug) producing endophytic fungi: An overview. Int J Pharma Bio Sci. 2010, 1: 1-9.
[33] Zhao J, Shan T, Mou Y and Zhou L. Plant-Derived Bioactive Compounds Produced by Endophytic Fungi. Mini-Reviews in Medicinal Chemistry, 2011, 11: 159-168.
[34] Schiff PB, Fant J and Horwitz SB. Promotion of microtubule assembly in vitro by taxol. Nature, 1979, 277: 665-667.
[35] Wang LG, Liu XM, Kreis W and Budman DR. The effect of antimicrotubule agents on signal transduction pathways of apoptosis: a review. Cancer Chemother. Pharm., 1999, 44: 355-361.
[36] Sadananda TS, Nirupama R, Chaithra K, Govindappa M, Chandrappa CP and Vinay Raghavendra B. Antimicrobial and antioxidant activities of endophytes from Tabebuia argentea and identification of anticancer agent (lapachol). J Med Plant Res., 2011,5: 3643-52.
[37] Yu SF, Chen TM and Chen YH. Apoptosis and necrosis are involved in the toxicity of Sauropus androgynus in an in vitro study. J Formos Med Assoc., 2007, 106: 537-47.
[38] Türk M, Kaya B, Menemen Y and Oğuztüzün S. Apoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla L. species on HeLa cells in vitro. J Med Plants Res., 2011, 5: 4566-71.
[39] Caruso M, Colombo AL, Fedeli L, Pavesi A, Quaroni S, Saracchi M and Ventrella G. Isolation of endophytic fungi and actinomycetes taxane producers. Ann. Microbiol., 2000, 50: 3–14.
[40] Guo B, Li H and Zhang L. Isolation of a fungus producting vinblastine. J. Yunnan Univ. Nat. Sci., 1998, 20: 214–215.
[41] Ya-Tuan M, Li-Rui Q, Wen-Quan S, An-Ling Z and Jin-Ming G. Metabolites produced by an endophyte Alternaria alternata isolated from Maytenus hookeri.Chem.Nat.Compd., 2010, 46 (3).
[42] Zaferanloo B, Quang T D, Daumoo S, Ghorbani MM, Mahon PJ and Palombo EA. Optimization of protease production by endophytic fungus, Alternaria alternata isolated from an Australian native plant. World J. Microbiol. Biotechnol., 2014, 30(6): 1755-1762.
[43] Eyberger AL, Dondapati R and Porter JR. Endophyte fungal isolates from Podophyllum peltatum produce podophyllotoxin. J. Nat. Prod., 2006, 69: 1121-1124.
[44] Cao L, Huang J and Li J. Fermentation conditions of Sinopodophyllum hexandrum endophytic fungus on production of podophyllotoxin. Food Fermentation Ind., 2007, 33: 28-32.
[45] Kour A, Shawl AS, Rehman S, Sultan P, Qazi PH, Suden P, Khajuria RK and Verma V. Isolation and identification of an endophytic strain of Fusarium oxysporum producing podophyllotoxin from Juniperus recurva. World J. Microbiol. Biotechnol., 2008, 24: 1115-1121.
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Published
2019-03-01
How to Cite
HOUALI, K.; OUZID, Y.; SAADOUN, N. S. ANTIMITOTIC AND ANTIPROLIFERATIVE ACTIVITIES OF CRUDE FUNGAL EXTRACTS OF ENDOPHYTIC FOLIAR FUNGI OF PEGANUM HARMALA L. FROM DAYATE AIAT (LAGHOUAT, ALGERIA). Journal of Fundamental and Applied Sciences, [S. l.], v. 11, n. 2, p. 587–604, 2019. DOI: 10.4314/jfas.v11i2.3. Disponível em: https://jfas.info/index.php/JFAS/article/view/190. Acesso em: 30 jan. 2025.
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