ANTAGONISM OF ISOLATES OF Trichoderma spp. at EGARD OF Botrytis cinerea Pers. AGENT OF GRAY ROT OF TOMATO (Lycopersicum esculentum Mill.) UNDER GREENHOUSE
DOI:
https://doi.org/10.4314/jfas.v12i2.5Keywords:
Trichoderma spp; Botrytis cinerea; activité antagoniste; Lycopersicum esculentum Mill; Prolex.Abstract
The study of the antagonistic activity of eleven isolates of Trichoderma spp., against four isolates of Botrytis cinerea, collected from diseased tomato plants cultivated in greenhouses located on the Algiers coast and in the north-east of Sahara. The in vitro antagonism was compared to the fungicidal activity of "PROLEX", widely used by farmers against this phytopathogen in Algeria. The results revealed a complete inhibition of sporulation, germination, production and germination of sclerotia as well as morphological changes in vitro. A significant reduction on their pathogenicity and a strong inhibition of their sporulation were recorded in vivo. In conclusion, the Trichoderma spp. antagonists in particular, the "T4, T11, T8 and T10" isolates are therefore promising for the biocontrol of grey mould of tomato.
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References
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[29] Yael M H, Zeraye H M, Dalia R D, Yigal E. Systemic Resistance to Gray Mold Induced in Tomato by Benzothiadiazole and Trichoderma harzianum T39. J. Phytopathology, 2014, 104 (2) , 150-177.
[30] O'Neill T M, Niv A, Elad Y, Shtienberg D. Biological control of Botrytis cinerea on tomato stem wounds with Trichoderma harzianum. European Journal of Plant Pathology, 1996, 102 (7), 635–643.
[31] Al Masri M I. Biological Control of Gray Mold Disease (Botrytis cinerea) on Tomato and Bean Plants by using local isolates of Trichoderma harzianum. Dirasat, Agricultural Sciences, 2014, 32 (2), 145-156.
[32] FAO, Eude de base sur la tomate en Algérie. Programme régional de gestion intégrée des ravageurs pour le Proche-Orient. GTFS/REM/070/ITA, Rome, Italie, 2010, pp. 52.
[33] Hmouni A, Mouria A, Douira A. Biological control of tomato grey mould with compost water extracts, Trichoderma sp., and Gliocladium sp. Phytopathologia Mediterranea, 2006, 45 (2), 110-116.
[34] Abd-El-Khair H, RKM K, Haggag K H E. Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. J .Am. Sci., 2010, 6 (9), 486-497.
[35] Bardin M, Ajouz S, Comby M, Lopez-Ferber M, Nicot P. Is the efficacy of biological control against plant diseases likely to be more durable than that of chemical pesticides?. Front. Plant. Sci., 2015, 6 (7), 566.
[36] Mouria B, Ouazzani TA, Douira A. In vitro and in vivo effects of compost on Verticillium dahliae, the causal agent of tomato Verticillium wilt. Bulletin de la Société Royale des Sciences de Liège, 2014, 83,10 – 34.
[37] Shedden K, Generalized Linear Models. Department of Statistics, University of Michigan. Creative Commons Attribution Share Alike 3.0 License, 2015, pp. 35.
[2] FAO, FAOSTAT-Crops- Tomato data., 2018, http://www.fao.org/faostat/en/#data/QC, (01/03/2018).
[3] Elmer P A G, Reglinski T. Bio-suppression of Botrytis cinerea in grapes. Plant Pathology., 2006, 55,155-177.
[4] Hmouni A, Oihabi L, Badoc A, Douira A. Study of resistance of Botrytis cinerea to benzimidazole, dicarboximide and dithiocarbamates in sheltered tomato crops in the Western region (Morocco). Bull. Soc. Pharm. Bordeaux., 2003, 142, 79-100.
[5] Hishkoff N, Mcgrath M T. AQ10 Biofungicide Combined with Chemical Fungicides or AddQ Spray Adjuvant for Control of Cucurbit Powdery Mildew in Detached Leaf Culture. Plant Dis., 2002, 86 (8), 915-918.
[6] Harman G. E., Howell C. R., Viterbo A., Chet I. and Lorito M., Trichoderma species opportunistic, avirulent plant symbionts. Nature Rev. Microbiol., 2004, 2(1), 43-56.
[7] Mukherjeev P K, Horwitz B A, Kenerley C M. Secondary metabolism in Trichoderma a genomic perspective. Microbiology, 2012, 158 (1), 35-45.
[8] Moumene S, Zanoune S, Laidani M, Bouznad Z. Biocontrol of Phytophthora infestans (Mont.) de Bary, The causal agent of potato late blight, with isolates of Trichoderma spp., medicinal plant-based preparations and Posidonia- based preparations. In Natural products and Bio-control. Presses Universitaires de Perpignan, France, 2015, pp.108.
[9] Messgo-Moumene S, Haddadj-Hamdi S, Saddek D, Zanoune S, Bellatreche M, Hamlaoui Y, Houmani Z. Essais de biocontrôle contre le rhizoctone brun de la pomme de terre, en Algérie : Effets antifongiques des extraits de plantes et effets antagonistes de quelques isolats du genre Trichoderma sur Rhizoctonia solani Kuhn. Séminaire International sur « Santé des Plantes au Service du Développement, Université de Chlef, Algérie. Communication Orale et Proceeding, 2016, pp. 216.
[10] Hibar K, Daami-Remadi M, Khiareddine H, El Mahdjoub M. Inhibitory effect in vitro and in vivo of Trichoderma harzianum on Fusarium oxysporum f. sp. radicis-lycopersici. Biotechnology, Agronomy, Society and Environment, 2005, 9 (3), 163-171.
[11] Krauss U, Soberanis W. Effect of fertilization and biocontrol application frequency on cocoa pod diseases. Biological Control, 2002, 24 (5), 82-89.
[12] Osman M E H, El-Sheekh M M, Metwally M A, Ismail A E A, Ismail M M. Antagonistic activity of some fungi and cyanobacteria species against R. solani. Int. J of Plant Pathology, 2011, 2 (3), 101-114.
[13] Bouziane Z, Dehimat I, Kacem N, Chaouche W, Abdel –Aziz W. La compétition de Trichoderma viride vis-à-vis des souches fongiques pathogènes de Zea mays. Sciences and Technologie, 2011, 33 (6), 31-37.
[14] Léopold S G, Dénis H, Tonon E, Euloge M, Faton O, Kobi O, Douro K, Dannon E, Akoegninou A. Efficacité de l’agent antagoniste Trichoderma harzianum sur Fusarium oxysporum f. sp. lycopersici agent pathogène de la tomate. Int. J. Biol. Chem. Sci. 2015, 9 (2), 770-782.
[15] Mouria B, Ouazzani T, Douira A. Effet du compost de Trichoderma harzianum sur la suppression de la verticiliose de la tomate. Journal of Applied Biosciens, 2013, 70, 5531-5543.
[16] Jaejung L, Joo-Hung H, Kim G H, Kim J J. The antagonistic properties of Trichoderma spp. inhabiting woods for potential biological control of wood-damaging fungi. Holzforschung, 2012, 66 (7), 883–887.
[17] Boumaaza B, Benkhelifa M, Belkhoudja M. Salinity Influence upon Activity of Trichoderma. Asian Journal of Plant Pathology, 2015, 9 (4), 158-166.
[18] Ajith P S, Lakshmidevi N. Effect of volatile and non-volatile compound from Trichoderma spp. against Colletotrichum capsici incitant of anthracnose on bell peppers. Nat. Sci., 2010, 8 (9), 265-269.
[19] Amin F, Razdan V K., Mohiddin F A, Sheikh K A, Bhat P A. Effect of volatile metabolites of Trichoderma species against seven fungal plant pathogens in-vitro. J. Phytol., 2010, 2 (10), 34-37.
[20] Melo1 I S, Faull J, Parasitism of Rhizoctonia solani by strains of Trichoderma spp. Sci. agric. , 2000, 57 (1) , 55-59.
[21] Mahiout D, Benzohra I. Antagonism of three Trichoderma species against Botrytis fabae and B. cinerea, the causal agents of chocolate spot. World Applied Sciences Journal, 2012, 17 (3), 278-283.
[22] Bendahmane B S, Mahiout D, Benzohra I E, Benkada M Y. Antagonism of three Species against Botrytis fabae and B. cinerea, the causal agents of Chocolate spot of Faba Bean (Vicia fabae L.) in Algeria. World Applied Sciences journal, 2012, 17 (3), 278-283.
[23] Ibarra-Medina V A, Ferrera-Cerrato R, Alarcón A, Larahernández M E, Valdez-Carrasco J M, Isolation and screening of Trichoderma strains antagonistic to Sclerotinia sclerotiorum and Sclerotinia minor. Revista mexicana de micología, 2010, 31, 53-63.
[24] Mpika J, Kebe B I, N’Guessan K F, Isolation and identification of indigenous microorganisms of cocoa farms in Cote d’Ivoire and assessment of their antagonistic effects against Phytophthora palmivora, the causal agent of the black pod disease. O.Grillo (Ed.), Biodiversity Loss in a Changing Planet, 2011. INTECH, 303-319.
[25] Berber F, Ouazzani-Touhami M, Badoc A, Douira A. In vivo antagonism of two Trichoderma against four species of pathogenic Bipolaris on sorghum. Bull. Soc. Pharm. Bordeaux, 2009,148, 93-114.
[26] Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Applied and Environmental Microbiology, 2005, 71(1), 363–370.
[27] St Leger R J, Wang C. Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests. Applied Microbiology and Biotechnology, 2010, 85 (4), 901–907.
[28] Sandhu S S, Sharma A K, Beniwal V, Boel G, Batra P., Kumar A, Jaglan S, Sharma A K, and Malhotra S. Myco-biocontrol of insect pests, factor involved, mechanism, and regulation. Journal of Pathogen., 2012, Article ID 126819, pp. 10 .
[29] Yael M H, Zeraye H M, Dalia R D, Yigal E. Systemic Resistance to Gray Mold Induced in Tomato by Benzothiadiazole and Trichoderma harzianum T39. J. Phytopathology, 2014, 104 (2) , 150-177.
[30] O'Neill T M, Niv A, Elad Y, Shtienberg D. Biological control of Botrytis cinerea on tomato stem wounds with Trichoderma harzianum. European Journal of Plant Pathology, 1996, 102 (7), 635–643.
[31] Al Masri M I. Biological Control of Gray Mold Disease (Botrytis cinerea) on Tomato and Bean Plants by using local isolates of Trichoderma harzianum. Dirasat, Agricultural Sciences, 2014, 32 (2), 145-156.
[32] FAO, Eude de base sur la tomate en Algérie. Programme régional de gestion intégrée des ravageurs pour le Proche-Orient. GTFS/REM/070/ITA, Rome, Italie, 2010, pp. 52.
[33] Hmouni A, Mouria A, Douira A. Biological control of tomato grey mould with compost water extracts, Trichoderma sp., and Gliocladium sp. Phytopathologia Mediterranea, 2006, 45 (2), 110-116.
[34] Abd-El-Khair H, RKM K, Haggag K H E. Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. J .Am. Sci., 2010, 6 (9), 486-497.
[35] Bardin M, Ajouz S, Comby M, Lopez-Ferber M, Nicot P. Is the efficacy of biological control against plant diseases likely to be more durable than that of chemical pesticides?. Front. Plant. Sci., 2015, 6 (7), 566.
[36] Mouria B, Ouazzani TA, Douira A. In vitro and in vivo effects of compost on Verticillium dahliae, the causal agent of tomato Verticillium wilt. Bulletin de la Société Royale des Sciences de Liège, 2014, 83,10 – 34.
[37] Shedden K, Generalized Linear Models. Department of Statistics, University of Michigan. Creative Commons Attribution Share Alike 3.0 License, 2015, pp. 35.
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Published
2020-03-15
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SADDEK, D.; MESSGO-MOUMENE, S.; CHEMAT-DJENNI, Z.; BENDIFALLAH, L.; BENCHEIKH, K. ANTAGONISM OF ISOLATES OF Trichoderma spp. at EGARD OF Botrytis cinerea Pers. AGENT OF GRAY ROT OF TOMATO (Lycopersicum esculentum Mill.) UNDER GREENHOUSE. Journal of Fundamental and Applied Sciences, [S. l.], v. 12, n. 2, p. 583–606, 2020. DOI: 10.4314/jfas.v12i2.5. Disponível em: https://jfas.info/index.php/JFAS/article/view/241. Acesso em: 30 jan. 2025.
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