IN VITRO ANTIBACTERIAL ACTIVITY OF CHLOROFORM, ETHYL ACETATE AND BUTANOL EXTRACTS OF LEAVES OF CITRULLUS LANATUS VAR. CRIMSON SWEET GROWN IN THE REGION OF OUARGLA, ALGERIA
DOI:
https://doi.org/10.4314/jfas.v11i2.30Keywords:
Citrullus Lanatus var. Crimson sweet, antibacterial activity, CMIAbstract
We are always trying to discover antibiotics, of natural origin, the most effective. For this purpose, our work is devoted to the evaluation of the antibacterial activity of the chloroformic, ethyl acetate and butanolic extracts of the leaves of citrullus lanatus crimson sweet variety cultivated in the region of Ouargla. This activity was tested against microorganisms derived from the reference strains gram positive (Staphylococcus aureus and Streptococcus sp) and gram negative (Escherichia coli and Pseudomonas aeuruginosa). All extracts showed a remarkable sensitivity against bacteria by giving inhibition diameters varying between 12 and 26 mm. The evaluation of the minimum concentration of inhibition (MIC) led to the values between 40 and 75 μg/ml. From the results obtained, we can conclude that the leaves of this plant have a good antibacterial activity against certain microorganisms.
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References
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[3] Kumawat G ,Goyal M, Math ur K, Yadav S K, Citrullus lanatus: An Overview on Pharmacological Activities, International Journal of Pharmaceutical & Biological Archives 2017; 8 (1), 6 – 9
[4] Siddiqui, W. A., Shahzad, M., Shabbir, A., and Ahmad, A. Evaluation of anti-urolithiatic and diuretic activities of watermelon (Citrullus lanatus) using in vivo and in vitro experiments. Biomedicine & Pharmacotherapy, 2018. 97, 1212-1221.
[5] Erhirhie, E., and Ekene, N.. Medicinal values on Citrullus lanatus (watermelon): pharmacological review. International Journal of Research in Pharmaceutical and Biomedical Sciences, 2013, 4(4), 1305-1312.
[6] Saboo, S. S., Thorat, P. K., Tapadiya, G. G., and Khadabadi S., Ancient and recent medicinal uses of cucurbitaceae family, International Journal of Therapeutic Applications, 2013, 9, 11-19.
[7] Biu, A. A., Buratai, L. B., Luka, J., Konto, M., Onwuatogwu, J., and Agada, N. O.. In vitro assessment of trypanocidal activity of aqueous extract of Citrullus lanatus (Cucurbitaceae)(Thunb) leaf and its effects on the haematological parameters of Trypanosoma brucei infected Albino rats. Biokemistri, 2013, 25(3), 127-132.
[8] Quezel P. and Santa S., Nouvelle flore de l'Algerie et des régions désertiques méridionales , Vol. II, Centre National de la Recherche Scientifique, Paris, 1963, pp. 894-895
[9] http: //www.dknews-dz.com/pdf/voir.html?id=1775 ; consulté le 02/12/2018
[10] Ho, L.-H., Ramli, N. F., Tan, T.-C., Muhamad, N., and Haron, M. N., Effect of Extraction Solvents and Drying Conditions on Total Phenolic Content and Antioxidant Properties of Watermelon Rind Powder. Sains Malaysiana, 2018, 47(1), 99-107
[11] Tlili, I., Hdider, C., Lenucci, M. S., Riadh, I., Jebari, H., and Dalessandro, G. Bioactive compounds and antioxidant activities of different watermelon (Citrullus lanatus (Thunb.) Mansfeld) cultivars as affected by fruit sampling area. Journal of Food Composition and Analysis, 2011, 24(3), 307-314.
[12] Cowan, M. M. Plant products as antimicrobial agents. Clinical microbiology reviews,1999 12(4), 564-582.
[13] Cemaluk, C. E. A., Comparative investigation of the antibacterial and antifungal potentials of the extracts of watermelon (Citrullus lanatus) rind and seed. EJMP, 2015, 9(4). 1-7.
[14] Rahman, H., Priyanka, P., Lavanya, P., Srilakshmi, N., & Kumar, P. R., A review on ethno botany, phytochemistry and pharmacology of Citrullus lanatus L. International Research Journal of Pharmaceutical and Applied Sciences, 2013., 3(2), 77-81.
[15] Chang S.-T., Wu J.-H., Wang S.-Y., Kang P.-L., Yang N.-S., and Shyur L.-F.,
J. Agric. Food Chem., 2001, 49 (7), 3420–3424, DOI: 10.1021/jf0100907
[16] Wang H.; Gao X. D.; Zhou G. C.; Cai, L.; Yao W. B., Food Chemistry 2008, 106, 888-895, doi :10.1016/j.foodchem.2007.05.068
[17] Toty, A. A., Guessennd, N., Bahi, C., KRA, A. K. M., Tokore, D. A., & Dosso, M. Évaluation in-vitro de l’activité antibactérienne de l’extrait aqueux de l’écorce de tronc de Harungana madagascariensis sur la croissance de souches multi-résistantes. Bulletin de la Société Royale des Sciences de Liège, 2013, 82, 12-21
[18] Abdel-Wahab S. I., Abdul A. B., Zhew K., and Fong, S. Antimicrobial and Free Radical Scavenging activities of the dichloromethane extract of Goniothalamus umbrosus. Int. J. Trop. Med, 2009, 4(1), 32-36
[19] Réseau Algérien de Sureillance de la Résistace des Bactéries aux Antibiotiques, Standardisation de l’antibiogramme à l’échelle nationale (médecine humaine et vétérinaire) Document édité avec la collaboration de l’OMS, 6ème Edition, 2011
[20] Bauer, A., Kirby, W., Sherris, J. C., and Turck, M. Antibiotic susceptibility testing by a standardized single disk method. Amer. J. clin. Pathol., 1966, 45(4), 493-496.
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Published
2019-04-29
How to Cite
BENNOUNA, A.; LABED, B.; DENDOUGUI, H. IN VITRO ANTIBACTERIAL ACTIVITY OF CHLOROFORM, ETHYL ACETATE AND BUTANOL EXTRACTS OF LEAVES OF CITRULLUS LANATUS VAR. CRIMSON SWEET GROWN IN THE REGION OF OUARGLA, ALGERIA. Journal of Fundamental and Applied Sciences, [S. l.], v. 11, n. 2, p. 1023–1032, 2019. DOI: 10.4314/jfas.v11i2.30. Disponível em: https://jfas.info/index.php/JFAS/article/view/391. Acesso em: 30 jan. 2025.
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