HS-SPME-GC/ MS ANALYSIS OF 3 LAMIACEAE PLANTS: AJUGA IVA (L.) SCHREB., SALVIA VERBENACA L. AND THYMUS ALGERIENSIS BOISS. & REUT.
DOI:
https://doi.org/10.4314/jfas.v12i2.12Keywords:
Ajuga iva; Salvia verbenaca; Thymus algeriensis; Lamiaceae.Abstract
The chemical analysis of three Algerian plants was conducted by using Headspace Solid-Phase Micro-Extraction coupled with Gas Chromatography-Mass Spectrometry (HS-SPME-GC/ MS). This analysis was performed directly on the dried aerial parts of the plants. The results revealed that the major compounds are 1,8-cineol (27.3%) in Ajuga iva, cis-muurola-3,5 diene (14.6%) in Salvia verbenaca and 2,3-dehydro-1,4-cineol (36%) in Thymus algeriensis. The oxygenated monoterpenes are the major class representing 62.1% and 78.4% in Ajuga iva and Thymus algeriensis, respectively, whereas the sesquiterpene hydrocarbons are the major class in Salvia verbenaca representing 50.1%. Identification of naturally rare monoterpene ether, 2,3-dehydro-1,4-cineol was the first time in the Ajuga iva,
Salvia verbenaca and Thymus algeriensis.
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References
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[25] Giordani R, Hadef Y, Kaloustian J. Composition and antifungal activities of essential oils of some Algerian aromatic plants. J Fitoterapia., 2008, 79:199-203.
[26] Dob T, Dahmane D, Benabdelkader T, Chelghoum C. Studies on the essential oil composition and antimicrobial activity of Thymus algeriensis Boiss. et Reut. Int J Aromather., 2006, 16: 95-100.
[27] Chemat S, Cherfouh R, Meklati BY, Belanteur K. Composition and microbial activity of Thyme (Thymus algeriensis genuinus) essential oil. J Essent. Oil Res., 2012, 24(1): 5-11.
[28] Al-Jaber HI. Essential oil composition of the aerial parts of fresh and air-dried Salvia verbenaca L. growing wild in Jordan. J Essent Oil-bear Plants, 2015, 18 (3):718-724.
[29] Saez F. Volatile oil variability in Thymus serpylloides ssp. gadorensis wild in sotheastern Spain. Biochem Syst Ecol., 2001, 29, 189-198.
[30] Pluhar Z, Sàrosi S, Novàk I, Kutta G. Essential oil polymorphism of Hungarian common thyme (Thymus glabrescens Willd.) populations. Nat Prod Commun. 2008, 3, 1151-1154.
[31] Skoula M, El Hilali I, Makris AM. Evaluation of the genetic diversity of Salvia fruticosa Mill. using RAPD markers and comparison with the essential oil profiles. Biochem Syst Ecol., 1999, 27, 559-568.
[2] FAO. Global forest resources assessment 2000. Main report. FAO forestry paper 140. FAO, Rome, Italy, 2001, 479 p.
[3] Sofowora A. Plantes médicinales et médecine traditionnelle. Académie suisse des sciences naturelles. Edition :Karthala, 2010, 171 p.
[4] Heinrich M, Barnes J, Gibbons S, Williamson EM. Fundamentals of pharmacognosy and phytotherapy. Second edition. Churchill livingstone Elsevier, 2012, 326 p.
[5] Roussef R, Cadwallader K. Headspace analysis of foods and flavors: theory andpractice. Springer Science and Buisiness Media, 2012, 212 p.
[6] Pawliszym J. Application of solid phase microextraction. Royal society of chemistry,1999, 654 p.
[7] Papet Y, Brunet B, Mura P. Headspace (HS) and solid phase micro-extraction (SPME). Theory and applications. J Ann Toxicol Anal. 2010, 22(2):75-79.
[8] Nikolić M, Glamočlija J, Ferreira I, Calhelha RC, Fernandes A, Marković T, Marković D, Giweli A, Soković M. Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L.,Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. Ind Crops Prod, 2014, 52, 183-190.
[9] Ayari B, Riahi L, Ziadi S, Chograni H, Mliki A. Evaluation of antioxidant and antimicrobial activities of Tunisian Ajuga iva. Essential oils. Revue f S B XI., 2013, 203-210.
[10] Canzoneri M, Bruno M, Rosselli S, Russo A, Cardile V, Formissano C, Rigano D, Senator F. Chemical composition and biological activity of Salvia verbenaca essential oil. Nat Prod Commun., 2011, 6 (7): 1023-1026.
[11] Asakawa Y, Ludwiczuk A, Sakurai K, Tomiyama K, Kawakami Y, Yaguchi Y. Comparative study on volatile compounds of Alpinia Japonica and Elettaria cardamomun. J Oleo Sci., 2017, 66. (8): 871-876.
[12] D’Auria M, Racioppi R. The effect of drying of the composition of volatile organic compounds in Rosmarinus officinalis, Laurus nobilis, Salvia officinalis and Thymus serpyllum. A HS-SPME-GC-MS Study. J Essent Oil-Bear Plants, 2015, 18 (5): 1209 – 1223.
[13] Meshkatalsadat MH, Heravi MRP, Rashidipour F. Chemical characterization of volatile organic components of Salvia officinalis using ultrasonic-assisted head space solid-phase microextraction and hydro-distillation extraction methods. J Univ Chem Technol Metallurgy, 2009, 44(3):291-296.
[14] Rzepa J, Wojtal L, Staszek D, Grygierczyk G, Labe K, Hajnos M, Kowalska T, Waksmundzka-Hajnos M. Fingerprint of selected Salvia species by HS–GC–MS analysis of their volatile fraction. J Chromatogr Sci., 2009, 47: 575-580.
[15] El-Sakhawy FS, Kassem HA, El-Gayed SH, Mostafa MM. Headspace Solid Phase Microextraction analysis of volatile compounds of the aerial parts and flowers of Plectranthus neochilus Schltr. And Salvia farinacea Benth. J Essent Oil-bear Plants, 2018, 21 (3): 674 – 686.
[16] Almeida V, Gonçalves V, Galego L, Miguel G, Costa M. Volatile constituents of leaves and flowers of Thymus mastichina by Headspace Solid-Phase Microextraction. J Acta Hortic., 2006, 723-730.
[17] Chouitah O, Meddah B, Aoues A, Sonnet P. Essential oil from the leaves of Ajuga iva: chemical composition and antimicrobial activity. J Essent Oil-bear Plants, 2017, 20(3): 873-877.
[18] EL-Hawary S, EL-Shabrawy A, Ezzat S, EL-Shibany F. Gas chromatography-mass spectrometry analysis, hepatoprotective and antioxidant activities of the essential oils of four Libyan herbs. J Med Plants Res., 2013, 7(24):1746-1753.
[19] Al-Howiriny T. Chemical composition and antimicrobial activity of essential oil of Salvia verbenaca. J Biotechnol., 2002, 1(1): 45-48.
[20] Hamdani I, Elouariachi E, Mokhtari O, Salki A, Chahboun N, Elmahi B, Bouyanzer A, Zarrouk A, Hammouti B, Costa J. Chemical constituents and corrosion inhibition of mild steel by the essential oil of Thymus algeriensis in 1.0 M hydrochloric acid solution. J Der pharma chem., 2015, 7(8): 252-264.
[21] El Ouariachi E, Hamdani I, Bouyanzer A, Hammouti B, Majidi L, Costa J, Paolini J Chetouani A. Chemical composition and antioxidant activity of essential oils of Thymus broussonetii Boiss. and Thymus algeriensis Boiss from Morocco. Asian Pac J Trop Dis., 2014, 4(4): 281-286.
[22] Benabed KH, Gourine N, Ouinten M, Bombarda I, Yousfi M. Chemical composition, antioxidant and antimicrobial activities of the essential oils of three Algerian Lamiaceae species. J Food Sci., 2017, 13(2): 97-109.
[23] Hazzit M, Baaliouamer A, Verissimo A R, Faleiro ML, Miguel MG. Chemical composition and biological activities of Algerian Thymus oils. J Food chem., 2009, 116: 714-721.
[24] Giweli AA, Dzamic AM, Sokovic MD, Ristic MS, Marin PD. Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya. Cent. Eur. J Biol., 2013, 8(5): 504-511.
[25] Giordani R, Hadef Y, Kaloustian J. Composition and antifungal activities of essential oils of some Algerian aromatic plants. J Fitoterapia., 2008, 79:199-203.
[26] Dob T, Dahmane D, Benabdelkader T, Chelghoum C. Studies on the essential oil composition and antimicrobial activity of Thymus algeriensis Boiss. et Reut. Int J Aromather., 2006, 16: 95-100.
[27] Chemat S, Cherfouh R, Meklati BY, Belanteur K. Composition and microbial activity of Thyme (Thymus algeriensis genuinus) essential oil. J Essent. Oil Res., 2012, 24(1): 5-11.
[28] Al-Jaber HI. Essential oil composition of the aerial parts of fresh and air-dried Salvia verbenaca L. growing wild in Jordan. J Essent Oil-bear Plants, 2015, 18 (3):718-724.
[29] Saez F. Volatile oil variability in Thymus serpylloides ssp. gadorensis wild in sotheastern Spain. Biochem Syst Ecol., 2001, 29, 189-198.
[30] Pluhar Z, Sàrosi S, Novàk I, Kutta G. Essential oil polymorphism of Hungarian common thyme (Thymus glabrescens Willd.) populations. Nat Prod Commun. 2008, 3, 1151-1154.
[31] Skoula M, El Hilali I, Makris AM. Evaluation of the genetic diversity of Salvia fruticosa Mill. using RAPD markers and comparison with the essential oil profiles. Biochem Syst Ecol., 1999, 27, 559-568.
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Published
2020-04-15
How to Cite
KHEMKHAM, A.; BELHADJ, S.; MEDDOUR, R.; KENMOKU, H.; AISSAOUI, R.; GOURINE, N.; YOUSFI, M.; HAKEM, A.; ASAKAWA, Y. HS-SPME-GC/ MS ANALYSIS OF 3 LAMIACEAE PLANTS: AJUGA IVA (L.) SCHREB., SALVIA VERBENACA L. AND THYMUS ALGERIENSIS BOISS. & REUT. Journal of Fundamental and Applied Sciences, [S. l.], v. 12, n. 2, p. 700–711, 2020. DOI: 10.4314/jfas.v12i2.12. Disponível em: https://jfas.info/index.php/JFAS/article/view/734. Acesso em: 30 jan. 2025.
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