POTENTIAL IN VITRO INHIBITORY EFFECTS OF MORINGA OLEIFERA LEAF EXTRACTS ON EXTENDED-SPECTRUM β-LACTAMASE-PRODUCING BACTERIA
DOI:
https://doi.org/10.4314/jfas.v13i1.8Keywords:
Moringa oleifera, antibacterial effect, phytochemicals, ESβL-producing bacteria, antimicrobial resistance.Abstract
Extended-spectrum β-lactamase (ESβL) enzymes are produced by certain bacteria as a mechanism of resistance to β-lactam and extended-spectrum β-lactam antibiotics. Previous investigations have confirmed that M. oleifera contains several bioactive compounds. This study aimed at evaluating the in vitro antibacterial effects of ethanolic and methanolic leaf extracts of M. oleifera on extended-spectrum β-lactamase producing bacteria. The leaf extracts of the plant were prepared, screened for bioactive compounds and evaluated for in vitro inhibitory effects against the ESβL-producing bacteria using agar well diffusion method. Different phytochemicals were detected from the extracts. Both methanolic and ethanolic leaf extracts showed a high inhibitory activity, which increased with an increase in concentration, from lowest to highest based on the zones of growth inhibition produced. Amongst the test organisms, Klebsiella pneumoniae was the most sensitive isolate to methanolic extract at 200, 100, 50 and 25 mg/mL followed by Escherichia coli, and then Proteus mirabilis. However, in terms of the ethanolic extract, using the same concentrations as those of methanol extracts, E. coli was found to be the most sensitive isolate followed by K. pneumoniae and then P. mirabilis.
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References
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parasitology, 2005.
[2] Centers for Disease Control and Prevention, Office of Infectious Disease. Antibiotic resistance threats in the United States, 2013. April 2013. Available at: http://www.cdc.gov/drugresistance/ threat-report-2013. Accessed January 28, 2015.
[3] Spellberg B, Gilbert DN. The future of antibiotics and resistance: a tribute to a career of leadership by John Bartlett. Clin Infect Dis 2014;59 suppl 2:S71–S75.
[4] Viswanathan VK. Off-label abuse of antibiotics by bacteria. Gut Microbes 2014;5(1):3–4.
[5] Read AF, Woods RJ. Antibiotic resistance management. Evol Med Public Health 2014;2014(1):147.
[6] Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and therapeutics, 2015, 40(4), 277.
[7] Garba L, Yusha’u M, Abdullahi MM, Abubakar MU, Inuwa AB, Isa S, Adamu MT. Effectiveness of double discs synergy test in the confirmation of extended spectrum β-lactamase (ESβL) Production. Journal of Biochemistry, Microbiology and Biotechnology, 2018, 6(2), 15-18.
[8] Wang L, Chen X, Wu A. Mini review on antimicrobial activity and bioactive compounds of Moringa oleifera. Med. Chem, 2016, 6, 578-582.
[9] Othman AS, Ahmed NA. Antibacterial Effect of the Ethanol Leaves Extract of Moringa oleifera and Camellia sinensis against multi drug resistant bacteria. International Journal of Pharmacology, 2017, 13(2), 156-165.
[10] Sayeed MA, Hossain MS, Chowdhury MEH, Haque M. In vitro antimicrobial activity of methanolic extract of Moringa oleifera Lam. fruits. Journal of Pharmacognosy and Phytochemistry, 2012, 1(4), 94.
[11] Guediri I, Boubekri C, Smara O. Preliminary phytochemical screening from different extracts of Solanum nigrum plant growing in south of algeria. J Fundam Appl Sci. 2020, 12(2), 624-633
[12] Haoulia A. Tests phytochimiques, dosage et recherche d’effet hémolytique des polyphénols totaux extraits de la partie aérienne d’Ammoïdes verticillata (Doctoral dissertation), 2015.
[13] Idris, A., and Abubakar, U. Phytochemical and antibacterial investigations of moringa (Moringa oleifera) leaf extract on selected bacterial pathogens. Journal of Microbiology and Antimicrobials, 2016,8(5), 28-33.
[14] Arévalo-Híjar, L., Aguilar-Luis, M. Á., Caballero-García, S., Gonzáles-Soto, N., and Valle-Mendoza, D. Antibacterial and cytotoxic effects of Moringa oleifera (moringa) and Azadirachta indica (neem) methanolic extracts against strains of Enterococcus faecalis. International journal of dentistry, 2018.
[15] Falowo, A. B., Muchenje, V., Hugo, C. J., and Charimba, G. In vitro antimicrobial activities of Bidens pilosa and Moringa oleifera leaf extracts and their effects on ground beef quality during cold storage. Cyta-Journal of Food, 2016, 14(4), 541-546.
[16] Peixoto JRO, Silva GC, Costa RA, Vieira GHF, Fonteles Filho AA, dos Fernandes Vieira RHS. In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts. Asian Pacific journal of tropical medicine, 2011, 4(3), 201-204.
[17] Moyo B, Masika PJ, Muchenje V. Antimicrobial activities of Moringa oleifera Lam leaf extracts. African Journal of Biotechnology, 2012, 11(11), 2797-2802.
[18] Uprety Y, Asselin H, Dhakal A, Julien N. Traditional use of medicinal plants in the boreal forest of Canada: review and perspectives. Journal of ethnobiology and ethnomedicine, 2012, 8(1), 7.
[19] Ibrahim Y, Sani Y, Saleh Q, Saleh A, Hakeem G. Phenotypic detection of extended spectrum beta lactamase and carbapenemase co-producing clinical isolates from two tertiary hospitals in Kano, North West Nigeria. Ethiopian journal of health sciences, 2017, 27(1), 3-10.
[20] Garba L, Yusha’u M. Detection of Extended-Spectrum Β-Lactamases among Gram Negative Isolates from Gombe Specialist Hospital Using Disc Replacement Method. Bayero Journal of Pure and Applied Sciences, 2012, 5(1), 109-112.
[21] Shu’aibu I, Hadiza JA, Yusha’u M, Kabiru MY, Ahmad MM, Lawal G, Khairiyya M. Assessment of foods and drinks for the presence of extended spectrum beta lactamase (ESBL) producing bacteria in Gombe Metropolis, Nigeria. Advanced Science Letters, 2018, 24(5), 3646-3651.
[22] Garba L, Lawan HS, Yusuf I, Abdullahi MM, Mukhtar MD, Puma HU Phytochemical Screening and in vitro Bacteriostatic Effects of Syzigium aromaticum (Clove) Extracts on Clinical Bacterial Isolates. Journal of Biochemistry, Microbiology and Biotechnology, 2019, 7(1).
[23] Otter JA, Yezli S, Salkeld JA, French GL. Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings. American journal of infection control, 2013, 41(5): 6-11.
[24] Yusha’u M, Garba L, Shamsuddeen U. In vitro inhibitory activity of garlic and ginger extracts on some respiratory tract isolates of gram-negative organisms. International Journal of Biomedical and Health Sciences, 2008, 4(2).
[25] Harborne JB. Phenolic compounds. In Phytochemical methods, 1973, (pp. 33-88). Springer, Dordrecht.
[26] Brain KR, Turner TD. The practical evaluation of phytopharmaceuticals, 1975, (Vol. 1). Bristol: Wright-Scientechnica.
[27] Garba, L., Chiroma, N. M., Justine, J., Yusuf, I., Inuwa, A. B., and Idris, A. Phenotypic Detection of Extended Spectrum β-lactamase-producing Bacteria from Selected Hospital Contact Surfaces. Journal of Environmental Microbiology and Toxicology, 2020, 8(1), 32-36.
[28] Jarlier V, Nicolas MH, Fournier G, Philippon A. Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Clinical Infectious Diseases, 1988, 10(4): 867-878.
[29] Cheesebrough M. Medical laboratory manuals for tropical countries, microbiology and
parasitology, 2005.
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Published
2020-10-01
How to Cite
GARBA, L.; MUHAMMAD, M. B.; ADAMU, M.; ISA, S.; ABDULLAHI, M. M.; YARMA, A. A. POTENTIAL IN VITRO INHIBITORY EFFECTS OF MORINGA OLEIFERA LEAF EXTRACTS ON EXTENDED-SPECTRUM β-LACTAMASE-PRODUCING BACTERIA. Journal of Fundamental and Applied Sciences, [S. l.], v. 13, n. 1, p. 137–150, 2020. DOI: 10.4314/jfas.v13i1.8. Disponível em: https://jfas.info/index.php/JFAS/article/view/869. Acesso em: 30 jan. 2025.
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