FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITYN OF DIFFERENT ORGANIC CRUDE EXTRACTS OF THE STEM OF SOLANUM NIGRUM L. BY ELECTROCHEMICAL METHOD
DOI:
https://doi.org/10.4314/jfas.v13i2.15Keywords:
Solanum nigrum L.; stem; crude extract; total flavonoids; antioxidant activity; cyclic voltammetry.Abstract
The present research aimed to quantify the total flavonoid content and antioxidant properties in the different crude extract of the stem of Solanum nigrum that grow in southern Algeria. The results showed that EtOAc extract showed the highest flavonoid content (18.410±0.023 mg QE/g). The electrochemical behavior of the chloroform, ethyl acetate and n-butanol extracts was studied and indicated that all extracts were electroactive and possess antioxidant activity, chloroform extract showed the highest capacity (9.58±0.010 mg/g). it has been shown that these samples showed scavenging ability on superoxide anion produced by electrochemical reduction of oxygen. The chloroform extract was the most efficient extract presenting the lowest IC50 values (0.664 mg/mL). The binding constant (Kb) of reaction and the change in free energy (ΔG) were calculated.
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References
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[29] Feng Q, Li NQ and Jiang YY. Electrochemical studies of porphyrin interacting with DNA and determination of DNA. Analytica Chimica Acta, 1997, 344, 97-104.
[30] Ahmed S and Shakeel F. Voltammetric determination of antioxidant character in Berberis lycium Royel, Zanthoxylum armatum and Morus nigra Linn plants. Pakistan Journal of Pharmaceutical Sciences, 2012, 25(3), 501-507.
[2] Saad-Allah KM and Elhaak MA. Hyperaccumulation activity and metabolic responses of Solanum nigrum in two differentially polluted growth habitats. Journal of the Saudi Society of Agricultural Sciences, 2017, 16, 227–235.
[3] kalwar K and Rizvi SAK. Estimation of total flavonoids contents and antibacterial activity from varions parts of Solanum nigrum L. Science International, 2016, 28(4), 3935-3937.
[4] Chester K, Zahiruddin S, Ahmad A, Khan W, Paliwal S and Ahmad S. Bioautography-based identification of antioxidant metabolites of Solanum nigrum L. and exploration its hepatoprotective potential against D-Galactosamine-induced hepatic fibrosis in rats. Pharmacognosy Magazine, 2019, 15(62), 104s-110s.
[5] Campisi A, Acquaviva R, Raciti G, Duro A, Rizzo M and Santagati NA. Antioxidant activities of Solanum nigrum L. leaf extracts determined in In Vitro cellular models. Foods, 2019, 8(63), 1-12.
[6] Gbadamosi IT and Afolayan AJ. In vitro anti-radical activities of extracts of Solanum nigrum (L.) from South Africa. Journal of Applied Biosciences, 2016, 9240- 9251.
[7] Maharana L, Kar DM, Pattnaik S, Sahu PK and Si SC. In Vitro antioxidant activity of aqueous leaf extract of Solanum nigrum Linn. Pharmacology online, 2010, 3, 333-345.
[8] Bahorun T, Aumjaud E, Ramphul H, Rycha M, Luximon-Ramma A, Trotin F, and Aruoma OI. Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung, 2003, 47, 191-198.
[9] Barbosa GB, Gomez EC and Inutan EDV. Cyclic voltammetry and spectrophotometric determination of antioxidant activities of selected Ginger Species. Asian Journal of Biological and Life Sciences, 2018, 7(3), 98-104.
[10] Boutarfaia A, Bechki L, Lanez T, Lanez E, Kadri M. Synthesis, Antioxidant Activity, and Determination of Binding Parameters of Meso-Tetra-4-Actophenyl-Porphyrin and its Palladium (II) Complex with Superoxide Anion Radicals. Current Bioactive Compounds, 2020, 16(7), 1063 – 1071, https://doi.org/10.2174/1573407215666191017105239
[11] Lanez T, Henni M. Antioxydant activity and superoxide anion radical interaction with 2-(ferrocenylmethylamino)benzonitrile and 3-(ferrocenylmethylamino)benzonitrile. Journal of the Iranian Chemical Society, 2016, 13(9), 1741–1748, https://doi.org/10.1007/s13738-016-0891-1
[12] Lanez E, Bechki L and Lanez T. Antioxidant activities, Binding parameters and electrochemical behavior of superoxide anion radicals twords 1-Ferrocenylmethylthymine and 1-Ferrocenylmethylcytosine. Current Physical Chemistry, 2020, 10, 10-22.
[13] Lanez T, Henni M. Spectrophotometrical study of antioxidant standards interacting with 2,2-diphenyl-1-picrylhydrazyl radical. hemistry & Chemical Technology. 2016, 10(3), 255–258.
[14] Lanez T, Henni M. Determination of binding parameters of 2-(ferrocenylmethyl-amino)benzonitrile and 3-(ferrocenylmethylamino)benzo-nitrile with 2,2-diphenyl-1-picrylhydrazyl free radical. J. Fundam. Appl. Sci. 2016, 8(1), 176-181, http://dx.doi.org/10.4314/jfas.v8i1.10
[15] Keffous F, Belboukhari N, Sekkoum K, Djeradi H, Cheriti A and Aboul-Enein HY. Determination of the antioxidant activity of Limoniastrum feei aqueous extract by chemical and electrochemical methods. Cogent Chemistry, 2016, 2, 1186141.
[16] Veerapagu M, Jeya KR, Sankaranarayanan A and Rathika A. In vitro antioxidant properties of methanolic extract of Solanum nigrum L. fruit. The Pharma Innovation Journal, 2018, 7(5), 371-374.
[17] Abdelhadi OMA, Babiker SA, Bauchart D, Listrat A, Reond D, Hocquette JF and Faye B. Effect of gender on quality and nutritive value of dromedary camel (Camelus dromedarius) Longissimus Lumborum muscle. Journal of the Saudi Society of Agricultural Sciences, 2017, 16, 242-249.
[18] Morshed MN, Al Azad S, Deb H, Islam A and Shen X. Eco-friendly UV blocking finishes extracted from Amaranthus viridis and Solanum nigrum . Tekstilec, 2018, 61(2), 93-100.
[19] Hary R, Vasuki R, Anbu J, Muralikrishna B, Manasa G and Geethaniali. Comparative free radical scavenging and analgesic avtivity of ethanolic leaves and stem extracts of Solanum nigrum. Journal of Medical Sciences, 2013, 13(5), 327-336.
[20] Hameed A and Akhtar N. Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries. Acta Pharmaceutica, 2018, 68, 47-60.
[21] Sriram Prasath G, Archana P, Srinivasan PT and Subramanian S. Studies on the phytochemical screening and free radical scavenging potentials of Solanum nigrum leaves extract. Asian Journal of Pharmaceutical Clinical Research, 2016, 9(6), 316-321.
[22] Alam MN, Roy S, Anisuzzaman SM and M Rafiquzzaman. Antioxidant activity of the ethanolic extracts of leaves, stems and fruits of Solanum nigrum. Pharmacognosy Communications, 2012, 2(3), 67-71.
[23] Padmashree A, Sharma GK, Semwal AD and Mahesh C. Antioxygenic activity of Solanum nigrum L. Leaves in sunflower oil model system and its thermal stability. Food and Nutrition Sciences, 2014, 5, 1022-1029.
[24] Mohy-Ud-Din A, Khan Z-U-D, Ahmad M, Kashmiri MA, Yasmin S and Mazhar H. Chemotaxonomic significance of flavonoids in the Solanum nigrum complex. Journal of the Chilean Chemical Society, 2009, 54(4), 486-490.
[25] Ghasemzadeh A, Jaafar HZE and Rahmat A. Effects of solvent type on phenolics and flavonoids content and antioxidant activities in two varieties of young ginger (Zingiber officinale Roscoe) extracts. Journal of Medicinal Plants Research, 2011, 5(7), 1147-1154.
[26] Chevion S and Chevion M. Antioxidant status and human health: Use of cyclic voltammetry for the evaluation of the antioxidant capacity of plasma and of edible plants. Annals of the New York Academy of Sciences, 2000, 899(1), 308-325.
[27] Leite KCDS, Garciaa LF, Lobóna GS, Thomaza DV, Gonc EK¸ Morenoa A, Carvalhoa MFD, Rochaa ML, Santosb WTPD and Gil EDS. Antioxidant activity evaluation of dried herbal extracts: an electroanalytical approach. Revista Brasileira de Farmacognosia, 2018, 28, 325–332.
[28] Menezes Peixoto CRD, Fraga S, Rosa Justim JD, Gomes MS, Carvalho DG, Jarenkow JA and Moura NFD. Voltammetric determination of total antioxidant capacity of Bunchosia glandulifera tree extracts. Journal of Electroanalytical Chemistry, 2017, 799, 519–524.
[29] Feng Q, Li NQ and Jiang YY. Electrochemical studies of porphyrin interacting with DNA and determination of DNA. Analytica Chimica Acta, 1997, 344, 97-104.
[30] Ahmed S and Shakeel F. Voltammetric determination of antioxidant character in Berberis lycium Royel, Zanthoxylum armatum and Morus nigra Linn plants. Pakistan Journal of Pharmaceutical Sciences, 2012, 25(3), 501-507.
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Published
2021-03-01
How to Cite
GUEDIRI, I.; BOUBÉKRI, C.; SMARA, O. FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITYN OF DIFFERENT ORGANIC CRUDE EXTRACTS OF THE STEM OF SOLANUM NIGRUM L. BY ELECTROCHEMICAL METHOD. Journal of Fundamental and Applied Sciences, [S. l.], v. 13, n. 2, p. 914–930, 2021. DOI: 10.4314/jfas.v13i2.15. Disponível em: https://jfas.info/index.php/JFAS/article/view/1017. Acesso em: 30 jan. 2025.
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