INVOLVEMENT OF ABIOTIC STRESS TOLERANCE MECHANISMS IN BIOTIC STRESS TOLERANCE IN DURUM WHEAT
DOI:
https://doi.org/10.4314/jfas.v12i2.15Keywords:
Heat shock, antifungal activity, penicillium, powdery mildew, durum wheatAbstract
The objective of our study is to implicate the mechanisms of tolerance to abiotic stress by the synthesis of metabolites in tolerance to biotic stress. The extracted metabolites; proline, sugars and polyphenols from durum wheat seedlings subjected to heat shock (40°C), used to test antifungal activity on two fungal strains, powdery mildew and penicillium, under controlled conditions. The boussellam variety is more tolerant of applied stress than the Ciccio and Vitron varieties. The concentration of the three osmolytes varies from one variety to another; it increases in genotypes stressed compared to controls. Antifungal activity results in the appearance of an inhibition zone around the disc impregnated with the studied extract. Sugars have proven to be a highly effective antifungal agent compared to proline and polyphenols with maximum values (28,33 ± 2 mm) in oidium and (29 ± 1 mm) in penicillium.
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References
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[3] Lutts S, Kinet JM, Bouharmont J. NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann. Bot., 1996, 78: 389-398.
[4] Rosa M., Prado C, Podazza G, Hilal M, Interdonato R. Soluble sugars - Metabolism, sensing and abiotic stress. Plant signaling and behaviour., 2009, 4, 5, 388-393.
[5] Fiala V, Boutin JP, Barry P & Derridj S. Les métabolites de surface foliaire (phylloplan): présence et rôle dans les relations plante-insecte., 1993, Acta Botanica Gallica, 140:2, 207-216, DOI: 10.1080/12538078.1993.10515583
[6] Hamli S, Labhilili M, Kadi K, Kabthan A E H, Tagouti M, Kamar M, Kanzeri R, Alyadini M and Bouzerzour H. Heat shock effects on fluorescence, membrane stability, chlorophyll content and metabolites accumulation in durum wheat (Triticum turgidum L. var. durum) seedlings and relationships with stress tolerance indices., 2015, Advances Environ Biol., 9: 116-125.
[7] Bates LS, Waldrem RP, Teare ID. Rapid determination of free proline for water stress studies. Plant and Soil., 1973, 39: 205-208.
[8] Dubois M, Gilles KA, Hamilton JK, Rebers PT and Smith F. Clorometric method for determination of sugars and related substances. Analyical chemistry., 1956, 28(3), 35-356
[9] Sultana B, Anwar F, Rasi M, Chatha SAS. Antioxidant potential of extracts from different agro wastes: Stabilization of corn oil. Grasas Y Aceties., 2008, 59:205-217.
[10] Singleton VL, Ortorfer R and Lamnela RM. Analyse of total phenols and other oxidation substrate and antioxydants by mean of Folin Ciocalteu reagent. Academic press., 1999, 5:152-178
[11] Button B, Breton A, Fèvre M, Guy P, Larpent J P, Veau P. Moisissures utiles et nuisible. Importance industrielle.2ème éd. Revue et complete. Paris, Milan, Barcelonne : Masson., 1990, 512
[12] Chabasse, D. Les phaeohyphomycetes agents de phaeohyphomycoses: Des champignons emergents. Journalde mycology medicinal., 2002, 12 (2),65-85
[13] Nccls. National committee for clinical Laboraatory Sandars. Performnce standards for antimicrobial disks susceptibility test., 1997.Sixth ed. Approved standard M2-A6, Wayne, PA.
[14] Santarmaria JM., MM. Ludlow, S. Fukai. Contribution of osmotic adjustment to grain yield in Sorghum bicolor L. under water-limited conditions: 1. Water stress before anthesis., 1990, Aust. J. Agric. Res., 41 : 5 1-65.
[15] Cheeseman LM. Mechanisms of sdinity tolerance in plants, Plant Physiol., 1988, 87: 547-550.
[16] Barnett, H.L. and Hunter, B.B. Illustrated Genera of Imperfect Fungi. 3rd Edition, Burgess Publishing Co., Minneapolis., 1972, 241 p.
[17] Claude M, Nicolas B. La stimulation des défenses naturelles des plantes : Un vaccin vegetal. Perspectives Agricoles., 2009, PA n° 355 - p 36 37.
[18] Sylvie D, Ingrid A, Nicholas, Birch E, Elad Y N. Lombarkia P, Couzi P, Pierre P Auger J, Les sucres solubles, une opportunité pourl’agriculture durable ? Des expérimentations menées sur maïs, tomate, pomme de terre et pommier. PHYTOMA - La Defense des végétaux., 2011, N° 640 janvier
[19] Zuckerman B.M. Nematode chemotaxis and possible mechanisms of host/prey recognition. Ann. Rev. of Phytopathol., 1984, 22 : 95-113
[20] Perri R N. Chemoreception in parasitic nematodes. Annual Review of Phytopathology., 1996, 34 : 181-199.
[21] Doehlemann G, Molitor F, Hahn M. Molecular and functional characterization of fructose specific transporter from the gray mod fungus Botrytis cinerea. Fungal genetics and biology., 2005, 42, 7, 601-610.
[22] Mahmoudi S, khali M, Mahmoudi N. Etude de l’extraction des composés phénoliques de différentes parties de la fleur d’artichaut Cynara scolymus L. Nature & Technologie., 2013, 9: 35-40
[23] Hernandez M A, Olmos E, Corpas F J, Sevila F and Del Rio L A. Salt induced oxidative stress in chloroplast of pea plants. Plant Science., 1995, 15(2), 151-167.
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Published
2020-04-28
How to Cite
HAMLI, S.; KADI, K.; BEKHOUCHE, I.; HARNAN, I.; ADDAD, D.; ABDELMALEK, A.; HARRAT, N. INVOLVEMENT OF ABIOTIC STRESS TOLERANCE MECHANISMS IN BIOTIC STRESS TOLERANCE IN DURUM WHEAT. Journal of Fundamental and Applied Sciences, [S. l.], v. 12, n. 2, p. 738–754, 2020. DOI: 10.4314/jfas.v12i2.15. Disponível em: https://jfas.info/index.php/JFAS/article/view/660. Acesso em: 30 jan. 2025.
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