EVALUATION OF THE ANTIBACTERIAL AND ANTICOAGULANT ACTIVITY OF PHENOLIC EXTRACTS OF LINUM USITATISSIMUM L.
DOI:
https://doi.org/10.4314/jfas.v12i2.10Keywords:
Linum usitatissimum L., anticoagulant activity, polyphenol, TCK, TQ.Abstract
The objective of this study is to evaluate the phytochemical constituents and anticoagulant, and antibacterial activities of phenolic extracts obtained from flaxseed. The quantitative estimation of total phenols by the colorimetric method showed that both aqueous and methanolic extracts are low in these compounds. The evaluation of the antibacterial activity of polyphenols was carried out by the disc method by determining the minimum inhibitory concentration (MIC). The results showed that no antibacterial activity with polyphenols was observed; against the five bacteria tested.The anticoagulant activity of polyphenols was also evaluated in vitro using the cephaline-kaolin (TCK) and Quick time (TQ) tests. The coagulation times obtained on normal plasma indicate that they have moderate activity on both coagulation pathways.
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References
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[31] Mieszczanek J., Harrison L. M., Vlasuk G.P., Cappello M. Anticoagulantpeptides from Ancylostomacaninumare immunologically distinct and localize to Separate structures within the adult hookworm. Molecular and Biochemical Parasitology, 2004. 133: 319-323.
[32] Zhou H.Y., Hong J.L., Shu P., Juan Ni Y., Qin M. J. A new coumarin andAnticoagulant activity from Viola yedoensis Makino. Fitoterapia, 2009. 80:283–285.
[33] Bae J.Antithrombotic and profibrinolytic activities of phloroglucinol.Food and ChemicalToxicology, 2011. 49: 1572-1577.
[2] Middleton E., Kandaswami C., Theoharides T.C. The effect of plant flavonoides on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol.Rev, 2000.52(4), p: 673-751.
[3] Tzang B. S., Yang S. F., Fu S. G., Yang H.C., Sun H. L., Chen Y.C. Effects of dietary flaxseed oil on cholesterol metabolism of hamsters. Food Chemistry, 2009.114: 1450-1455.
[4] Békro Y.A., Békroj A.M., Bouab.B., Trab F.H. and Ehilé E.E. EtudeEthnobotanique et Screnning phytochimique de Caesalpinia benthamiana. (Bai) Herend et Zarucchi (caesalpiniaceae). Rev. Sci. Nat, 2007. 4 (2) : p. 217-225.
[5] Kalla, A. Etude et valorisation des principes actifs de quelques plantes du sud algérien : Pituranthos scoparius, Rantherium adpressum et Traganum nudatum. Thèse de Doctorat en Sciences, 2012.Université de Mentouri – Constantine
[6] Tadros S.H. Pharmacognostical study of entrolobuim cyclocarpum griseb growing in Egypt.Ph. D.thesis. Faculty of pharmacy.Cairouniversity.Florida state horticultural society, 1979.426p.
[7] Oloyede O.I, Chemical profile of Unripe Pulp of Carica papaya, Pakistan journal of nutrition, 2005. 4, p. 379-381.
[8] Koffi N., Beugré K., Guédé N., Dossahoua T., Laurent A. Screening phytochimique de quelques plantes médicinales ivoiriennes utilisées en pays Krobou (Agboville, Côte –d’Ivoire), Sciences & Nature, 2009. Vol.6N°1 :1-15.
[9] Dohou N., Yamni K., Thahrouch S., Idrissi Hassani L. M., Badoc A., GmiraN. Screening phytochimique d’une endémique ibéro-Marocaine, Thynelaealythroides. Bull.Soc, Pharm. Bordeaux, 2003.142:61-78.
[10] Tresse E. et Evans W.C. Pharmacognosy. Billiaire, Ed. Tindall, London1987. 13, p. 61-62.
[11] Memelink J., Verpoort R., Kijne J.W. Organisation of jasmonate-responsive gene expression in alkaloid metabolism, Trends plant sci, 200 1. 6:212-219
[12] Diallo D. Ethno pharmacological survey of medicinal plants in Mali and phytochemical study of four of them: Glinus oppositifolius (Azoceae), Diospyros abyssinica (Ebenaceae), Entada africana (Minosaceae), Trichilia emetic (Meliaceae), 2000.Thèse de doctorat de recherche, Faculté des sciences de l’université de Lausanne Suisse.
[13] Catalano L, Franco I, De Nobili M, Leita L. Polyphenols in olive mill waste waters and their depuration plant effluents: a comparaison of the Folin-Ciocalteauand HPLC methods, Agrochimica, 1999. p :46
[14] Skerget, M., P. Kotnik, M. Hadolin, A.R. Hras, M. Simonic and Z. Knez. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities, Food Chem, 2005. 89: 191-198.
[15] Lafka T.I, Sinanoglou V, Lazos E. S. On the extraction and antioxidant activity of phenolic compounds from winery wastes, Food Chemistry, 2007. p : 46.
[16] Li H.B.,Cheng K.W., Wong C. C., Fan K. W., chen F., Tian Y. Evaluation ofantioxidant capacity and total phenolic content of different fraction of selected microalgae. Food Chemistry, 2007. 102, p.771-776.
[17] Hayes A. J. et Markovic B.Toxicity of australian essential oil Backhousia Citriodora (Lemon myrtle). Part1, Antimicrobial activity and in vitro cytotoxicity, Food Chem Toxicol, 2002. 40: 535-543.
[18] Najjaa H., Neffati M., Zouari, S. andAmmar E. Essential oil composition andAntibacterial activity of different extracts of Allium roseumL, a North African endomic species. C.R.Chimie, 2007. 10:820-826.
[19] Rizzo F, Papasouliotis K, Crawford E, Dodkin S, Cue S. Measurement of prothrombin time (PT) and activated partial thromboplastin time (APTT) on canine citrated plasma samples following different storage conditions. Research in Veterinary Science, 2008. 85: 166-170.
[20] Wang J, Zhanga Q, Zhang Z, Songa H, Li P. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 2010. 46: 6-12.
[21] ALachaherFatima Zohra. Effet de la supplémentation des graines brunes de lin sur le profil lipidique et les statuts redox et inflammatoire, chez les rats rendus diabétique par streptozotocine. Thèse de Doctorat, 2018.Université d’AhmedBenbella, Oran, Alger.
[22] ZafarJaved Khan., Naeem Ahmad Khan., ImranaNaseem., Shahab A. A Nami. Exploration of physicochemical potential of Linum usitatissimum L (Tukhm-e-Katan).Asian journal of pharmacy and pharmacology, 2019.5(3):551-558.
[23] Acket S. (2015). Implication du métabolisme carboné pour une production différentielle d’huile chez les plantes oléagineuses-Lin : modélisation des systèmes. Thèse de doctorat, Université de Technologie, Compiègne.
[24] Anwar F., Przybylski R. Effect of solvents extraction on total phenolics and antioxidant activity of extracts from fl axseed (Linum usitatissimum L.). Acta Sci. Pol., Technol. Aliment, 2012. 11(3), 293-301.
[25] Lee K.W., Kim Y.J., Lee H.J., Lee C.Y. Cacao has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. Food chemistry, 2003. 51: 7292-7295.
[26] (Ebrahimiet al., EbrahimiNS.Hadian J., Mirjalili MH., Sonboli A., YoucefZadi M.( 2008). Essential oil composition and antimicrobial activity of thymus caramanicus at different phonological stages. Food chemistry, 2008.110:927-931
[27] Miliauskas G., Venskutonis P.R., Van Beek TA, Screening of radical scavenging activity of some medicinal and aromatic plant extract. Food chemistry, 2004. 85:231-237.
[28] Nesbitt P.D., Lam Y., and Thomposo L.U. Human metabolism of mammalian lignan precursors in raw and processed flaxseed .American journal of clinical nutrition, 1999. 69:549-555.
[29] Caquet R.250 Examens de laboratoire : prescription et interprétation (9éme Ed. Masson (Paris); 2004. p. 388-389.
[30] Sakthipriya P. etVidhya R. Phytochemical and in-vitro thrombolytic Activity of Pergulariadeamia (forsk.) stem.World Journal of PharmaceuticalResearch, 2015.4 (5): 1325-1337.
[31] Mieszczanek J., Harrison L. M., Vlasuk G.P., Cappello M. Anticoagulantpeptides from Ancylostomacaninumare immunologically distinct and localize to Separate structures within the adult hookworm. Molecular and Biochemical Parasitology, 2004. 133: 319-323.
[32] Zhou H.Y., Hong J.L., Shu P., Juan Ni Y., Qin M. J. A new coumarin andAnticoagulant activity from Viola yedoensis Makino. Fitoterapia, 2009. 80:283–285.
[33] Bae J.Antithrombotic and profibrinolytic activities of phloroglucinol.Food and ChemicalToxicology, 2011. 49: 1572-1577.
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Published
2020-04-29
How to Cite
BOUKERIA, S.; MNASRI, S. R.; KADI, K.; BENBOTT, A.; BOUGUERIA, H.; BIRI, K.; LAZBBACHE, W. EVALUATION OF THE ANTIBACTERIAL AND ANTICOAGULANT ACTIVITY OF PHENOLIC EXTRACTS OF LINUM USITATISSIMUM L. Journal of Fundamental and Applied Sciences, [S. l.], v. 12, n. 2, p. 667–682, 2020. DOI: 10.4314/jfas.v12i2.10. Disponível em: https://jfas.info/index.php/JFAS/article/view/786. Acesso em: 30 jan. 2025.
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