• Anwuli U. Osadebe World Bank Africa Centre of Excellence in Oilfield Chemicals Research, University of Port Harcourt, Nigeria
  • Ibiso W. Davis Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Choba, Nigeria
  • Chimezie J. Ogugbue Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Choba, Nigeria
  • Gideon C. Okpokwasili Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Choba, Nigeria



biochar, nitrogen cycle, petroleum, ecosystem services approach


This study adopted an ecosystem services approach to pollution management by investigating the impact of biochar-mediated remediation on soil nitrogen, abundance of nitrogen cycling bacteria and the activity of ammonia monooxygenase (AMO) enzyme in petroleum-polluted soil using two biochar types applied at two treatment levels with monitoring over 15 weeks. The corn cob-derived biochar (CDB), generally, had a stronger restorative effect on soil ammonium nitrogen, nitrate and total organic nitrogen concentrations than the bone-derived biochar (BDB). Both biochar types had a more robust impact on restoration of Nitrosomonas, Nitrobacter and Azotobacter abundance (with the re-establishment of pre-pollution levels) than on Rhizobium and Pseudomonas aeruginosa. Biochar amendment restored the activity of AMO enzyme in the soil by week 15. The CDB (72.4% – 73.7%) showed more effective total petroleum hydrocarbon (TPH) elimination capacity than the BDB (51.1% – 57.7%). Biochar amendments exhibited great potential for restoration of nitrogen cycling while facilitating remediation of petroleum-polluted soils.


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How to Cite

OSADEBE, A. U.; DAVIS, I. W.; OGUGBUE, C. J. .; OKPOKWASILI, G. C. . BIOCHAR-MEDIATED REMEDIATION IMPACTS ON NITROGEN CYCLING BACTERIA AND AMMONIA MONOOXYGENASE ACTIVITY IN CRUDE OIL POLLUTED SOIL. Journal of Fundamental and Applied Sciences, [S. l.], v. 14, n. 3, p. 466–489, 2022. DOI: 10.4314/jfas.1217. Disponível em: Acesso em: 28 nov. 2023.