BIOCHAR-MEDIATED REMEDIATION IMPACTS ON NITROGEN CYCLING BACTERIA AND AMMONIA MONOOXYGENASE ACTIVITY IN CRUDE OIL POLLUTED SOIL

Authors

  • Anwuli U. Osadebe World Bank Africa Centre of Excellence in Oilfield Chemicals Research, University of Port Harcourt, Nigeria
  • Ibiso W. Davis Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Choba, Nigeria
  • Chimezie J. Ogugbue Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Choba, Nigeria
  • Gideon C. Okpokwasili Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Choba, Nigeria

DOI:

https://doi.org/10.4314/jfas.1217

Keywords:

biochar, nitrogen cycle, petroleum, ecosystem services approach

Abstract

This study adopted an ecosystem services approach to pollution management by investigating the impact of biochar-mediated remediation on soil nitrogen, abundance of nitrogen cycling bacteria and the activity of ammonia monooxygenase (AMO) enzyme in petroleum-polluted soil using two biochar types applied at two treatment levels with monitoring over 15 weeks. The corn cob-derived biochar (CDB), generally, had a stronger restorative effect on soil ammonium nitrogen, nitrate and total organic nitrogen concentrations than the bone-derived biochar (BDB). Both biochar types had a more robust impact on restoration of Nitrosomonas, Nitrobacter and Azotobacter abundance (with the re-establishment of pre-pollution levels) than on Rhizobium and Pseudomonas aeruginosa. Biochar amendment restored the activity of AMO enzyme in the soil by week 15. The CDB (72.4% – 73.7%) showed more effective total petroleum hydrocarbon (TPH) elimination capacity than the BDB (51.1% – 57.7%). Biochar amendments exhibited great potential for restoration of nitrogen cycling while facilitating remediation of petroleum-polluted soils.

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References

Griffiths B, Philippot L. Insights into the resistance and resilience of the soil microbial community. FEMS Microbiology Reviews 2012; 37: 112 – 119.

MEA, Millennium Ecosystem Assessment. Ecosystems and Human Well-being: Current State and Trends. Findings of the Condition and Trends Working Group. Island Press, Washington, DC, USA; 2005.

Baskent EZ. A Framework for Characterizing and Regulating Ecosystem Services in a Management Planning Context. Forests 2020; 11 (102): 1 – 20.

Compton JE, Harrison JA, Dennis RL, Greaver TL, Hill BH, Jordan SJ, Walker H, Campbell HV. Ecosystem services altered by human changes in the nitrogen cycle: a new perspective for US decision making. Ecology Letters 2011; 14: 804 – 815.

Urakawa H, Garcia J, Barreto P, Molina G, Barreto J. A sensitive crude oil bioassay indicates that oil spills potentially induce a change of major nitrifying prokaryotes from the Archaea to the Bacteria. Environmental Pollution 2012; 164: 42 – 45.

USEPA, United States Environmental Protection Agency. Nitrification. U.S. Environmental Protection Agency, Office of Ground Water and Drinking Water, Standards and Risk Management Division, Washington, USA; 2002.

Narayana B, Sunil K. A Spectrophotometric Method for the Determination of Nitrite and Nitrate. Eurasian Journal of Analytical Chemistry 2009; 4(2): 204 – 214.

Halin S, Philippot L, Loffler E, Sanford RA, Jones CM. Genomics and Ecology of Novel N2O-Reducing Microorganisms. Trends in Microbiology 2018; 26(1): 43 – 55.

Robertson G, Groffman P. Nitrogen Transformations In: Eldor P., ed., Soil Microbiology and Biochemistry, 3rd Ed. Elsevier Academic Press, Amsterdam; 2007.

Urakawa H, Rajan S, Feeney ME, Sobecky PA, Mortazavi B. Ecological response of nitrification to oil spills and its impact on the nitrogen cycle. Environmental Microbiology 2019; 21(1), 18–33.

Kang YS, Park YJ, Jung J, Park W. Inhibitory effect of aged petroleum hydrocarbons on the survival of inoculated microorganism in a crude-oil-contaminated site. Journal of Microbiology and Biotechnology 2009; 19: 1672–1678.

Burdige DJ. Estuarine and coastal sediments coupled biogeochemical cycling. In: McLusky D, Wolans E, eds. Treatise on Estuarine and Coastal Science, Academic Press, Waltham, MA; 2012.

John RC, Ntino ES, Itah, AY. Impact of crude oil on soil nitrogen dynamics and uptake by legumes grown in wetland ultisol of the Niger Delta, Nigeria. Journal of Environmental Protection 2016; 7: 507 – 515.

Kanter DR. Nitrogen pollution: a key building block for addressing climate change. Climate Change 2018; 147, 11 – 21.

Bloem B, Breure A. Microbial Indicators. Chapter 2b, In: Markert B, Breure A, Zechmeister H, eds., Bioindicators and Biomonitors: Principles, Concepts and Applications. Volume 6, In: Nriagu J, ed., Trace Metals and other Contaminants in the Environment. Elsevier Science Limited, Oxford, UK; 2003.

Urakawa H, Bernhard AE. Wetland management using microbial indicators. Ecological Engineering 2017; 108: 456–476.

van den Belt M, Granek E, Gaill F, Halpern B, Thorndyke M, Bernal P. Assessment of Major Ecosystem Services from the Marine Environment (Other than Provisioning Services) Chapter 3. Scientific Understanding of Ecosystem Services Part III. United Nations; 2016.

Guo M, Song W, Tian J. Biochar-Facilitated Soil Remediation: Mechanisms and Efficacy Variations. Frontiers in Environmental Science 2020; 8: 521512.

Song L, Hou L, Zhang Y, Li Z, Wang W, Sun Q. Regular Biochar and Bacteria-Inoculated Biochar Alter the Composition of the Microbial Community in the Soil of a Chinese Fir Plantation. Forests 2020; 11: 951.

Tomcyzk A, Sokoloswka Z, Boguta P. Biochar physicochemical properties: pyrolysis temperature and feedstock kind effects. Reviews in Environmental Science and Biotechnology 2020; 19:191–215.

Noyce GL, Basiliko N, Fulthrope RR, Sackett TE, Thomas SC. Soil microbial responses over 2 years following biochar addition to north temperate forest. Biology and Fertility of Soils 2015; 51: 649 – 659.

Mitchell PJ, Simpson AJ, Soong R, Schurman JS, Thomas SC, Simpson MJ. Biochar amendment and phosphorus fertilisation altered forest soil microbial community and native soil organic matter molecular composition. Biogeochemistry 2016; 130: 227 – 245.

Ipppolito JA, Spokas KA, Novak JM, Lentz RD, Cantrell KB. Biochar elemental composition and factors influencing nutrient retention. In: Lehman J, Joseph S (eds.) Biochar for Environmental Management: Science, Technology and Implementation. 2nd Ed., Routledge, UK; 2015.

Morris MC, McMurdie HF, Evans EH, Paretzkin B, Parker HS, Wong-Ng W, Gladhill DM. Standard X-ray Diffraction Powder Patterns. NBS Monograph 25 – Section 21. U. S. Department of Commerce, National Bureau of Standards, Pennsylvania, USA; 1985.

Chen BL, Zhou D, Zhu L. Transitional adsorption and partition of nonpolar and polar aromatic contaminants by biochars of pine needles with different pyrolytic temperatures. Environmental Science and Technology 2008; 42: 5137 – 5143.

Mustapha AA, Abdu N, Oyinola EY, Nuhu AA. Comparison of 3 different methods of CEC determination in Nigerian savannah soils. Journal of Applied Sciences 2020; 20(4): 159 – 165.

Yuan JH, Xu RK, Zhang H. The forms of alkalis in the biochar produced from crop residues at different temperatures. Bioresource Technology 2011; 102: 3488 – 3497.

Maynard DG, Katra YP. Nitrate and exchangeable ammonium nitrogen. Chapter 4 In: Carter MR., ed., Soil Sampling and Methods of analysis. Canadian Society of Science, Lewis Publishers, Boca Raton, Florida, USA; 1993.

Keeney DR, Nelson DW. Nitrogen in organic form In: Page A., Ed., Methods of Soil Analysis, Part 2: Chemical and Microbiological Properties. American Society of Agronomy, Inc., Madison, Wisconsin USA; 1982.

Bremner JM. Nitrogen Total In: Sparks DL, Ed., Methods in Soil Analysis, Part 3, Chemical Methods. American Society of Agronomy Inc., Wisconsin, USA; 1996.

Holt GT, Krieg RN, Sneath PHA, Staley TJ, Williams TS. Bergey’s Manual of Determinative Bacteriology. 9th Ed. Williams and Wilkins, Baltimore, USA; 1994.

Cheesbrough M. District Laboratory Practice in Tropical Countries, Part II. Cambridge University Press, London, UK; 2006.

Vepsäläinen M. Poor enzyme recovery by extraction from soils. Soil Biology and Biochemistry 2001; 33: 1131 – 1135.

Šnajdr J, Valaskova V, Merhautova V, Herinkova J, Cajthaml T, Baldrin P. Spatial variability of enzyme activities and microbial biomass in the upper layers of Quercus petraea forest soil. Soil Biology and Biochemistry 2008; 40: 2068 – 2075.

Liu S, Vereecken H, Brüggemann N. A highly sensitive method for the determination of hydroxylamine in soils. Geoderma 2014; 232 – 234: 117 – 122.

USEPA, United States Environmental Protection Agency. Method 3560 – Supercritical extraction for total recoverable petroleum hydrocarbons (TRPHs). USEPA, Washington DC, USA; 1996.

Joseph S, Taylor P, Rezende F, Draper K, Cowie A. The Properties of Fresh and Aged Biochar. Armidale, NSW: Biochar for Sustainable Soils, Starfish Initiatives; 2019. Available at https://biochar.international/guides/properties-fresh-aged-biochar/, Accessed, 31st October, 2021.

IBI, International Biochar Initiative. Standardized Product Definition and Product Testing Guidelines for Biochar that is used in Soil – Version 2.1. Westerville, Ohio, USA: International Biochar Initiative; 2016.

EBF, European Biochar Foundation. European Biochar Certificate – Guidelines for a Sustainable Production of Biochar. European Biochar Foundation (EBF), Switzerland; 2012.

Ronsse F, van Hecke S, Dickinson D, Prins W. Production and characterisation of slow pyrolysis biochar: influence of feedstock type and pyrolysis conditions. GCB Bioenergy 2013; 5: 104 – 115.

Naeem MA, Khalid M, Arshad M, Ahmad R. Yield and nutrient composition of biochar produced from different feedstocks at varying pyrolytic temperatures. Pakistani Journal of Agricultural Science 2014; 51(1): 75 – 82.

Domingues RR, Trugilho PF, Silva CA, de Melo ICN, Melo LCA, Magriotis ZM, Sanchez-Monedero MA. Properties of biochar derived from wood and high-nutrient biomasses with the aim of agronomic and environmental benefits. PLoS ONE 2017; 12(5): e0176884.

Metcalf K. Biochar for Carbon Sequestration: Investigation and Outreach. Earth Science 2016; 32: 33 – 36.

Janu R, Mrlik V, Ribitsch D, Hofman J, Sedlacek P, Bielska L, Soja G. Biochar surface functional groups as affected by biomass composition and pyrolysis temperature. Carbon Resources Conversion 2021; 4: 36 – 46.

Wang S, Gao B, Zimmerman AR, Li Y, Ma L, Harris WG, Migliaccio KW. Physicochemical and sorptive properties of biochars derived from woody and herbaceous biomass. Chemosphere 2015; 134: 257 – 262.

Yang F, Lee X, Wang B. Characterization of biochars produced from seven biomasses grown in three different climate zones. Chinese Journal of Geochemistry 2015; 34: 592–600.

Mierzwa-Hersztek M, Gondek K, Baran A. Effect of poultry litter biochar on soil enzymatic activity, ecotoxicity and plant growth. Applied Soil Ecology 2016; 105:144–150.

Wertz S, Leigh A, Grayston S. Effects of long-term fertilization of forest soils on potential nitrification and on the abundance and community structure of ammonia oxidizers and nitrite oxidizers. FEMS Microbiology Ecology 2012; 79: 142 – 154.

Shetty R, Prakash NB. Effect of different biochars on acid soil and growth parameters of rice plants under aluminium toxicity. Scientific Reports 2020; 10: 12249.

Bista P, Ghimire R, Machado S, Pritchett L. Biochar effects on soil properties and wheat biomass vary with fertility management. Agronomy 2019; 9(10): 623.

Xu X, Liu W, Tian S, Wang W, Qi Q, Jiang P, Gao X, Li F, Li H, Yu H. Petroleum hydrocarbon-degrading bacteria for the remediation of oil pollution under aerobic conditions: A perspective analysis. Frontiers in Microbiology 2018; 9: 2885.

Huettel M, Overholt WA, Kostka JE, Hagan C, Kaba J, Wells WB, Dudley S. Degradation of Deepwater horizon oil buried in a Florida beach influenced by tidal pumping. Marine Pollution Bulletin 2018; 126: 488–500.

Van Dorst J, Siciliano SD, Winsley T, Snape I, Ferrari BC. Bacterial targets as potential indicators of diesel fuel toxicity in subantarcctic soils. Applied and Environmental Microbiology, 2014, 80(13), 4021 – 4033.

Okpokwasili GC, Odokuma LO. Tolerance of Nitrobacter to toxicity of some Nigerian crude oils. Bulletin of Environmental Contaminants and Toxicology 1993; 52: 388–395.

Okpokwasili GC, Odokuma, LO. Tolerance of Nitrobacter to toxicity of hydrocarbon fuels. Journal of Petroleum Science and Engineering 1996; 16: 89-93.

Zhao Y, Chen W, Wen D. The effect of crude oil on microbial nitrogen cycling in coastal sediments. Environment International 2020; 139: 105724.

Ameloot N, De Neve S, Jegajeevagan K, Yildiz G, Buchan D, Funkuin YN, Prins W, Bouckaert L, Sleutel S. Short term CO2 and N2O emissions and microbial properties of biochar amended sandy loam soils. Soil Biology and Biochemistry 2013; 57: 401–410.

Cao X, Harris W. Properties of dairy-manure-derived biochar pertinent to its potential use in remediation. Bioresource Technology 2010; 101 (14):5222–5228.

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Published

2022-09-01

How to Cite

OSADEBE, A. U.; DAVIS, I. W.; OGUGBUE, C. J. .; OKPOKWASILI, G. C. . BIOCHAR-MEDIATED REMEDIATION IMPACTS ON NITROGEN CYCLING BACTERIA AND AMMONIA MONOOXYGENASE ACTIVITY IN CRUDE OIL POLLUTED SOIL. Journal of Fundamental and Applied Sciences, [S. l.], v. 14, n. 3, p. 466–489, 2022. DOI: 10.4314/jfas.1217. Disponível em: https://jfas.info/index.php/JFAS/article/view/1217. Acesso em: 25 sep. 2022.

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