ANTIBACTERIAL ACTIVITY OF GVL, A LECTIN ISOLATED FROM MARINE ALGAE GRACILARIA VERRUCOSA

Authors

  • Ratheesh . Sadanandan Depatment of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India
  • Anoop Appu Depatment of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India
  • Arun. A. Rauf Depatment of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India

DOI:

https://doi.org/10.4314/jfas.1224

Keywords:

Marine algae, Gracilaria verrucosa, gel filtration chromatography, antibacterial activity, disc diffusion method.

Abstract

Lectins are univalent or polyvalent carbohydrate-binding proteins of non-immune origin that are widely distributed in the marine ecosystem. A lectin was isolated from marine algae Gracilaria verrucosa by G-100 gel filtration chromatography. This lectin was named GVL, agglutinated rat, rabbit, goat, chicken and human ABO erythrocytes. The antibacterial activity of this algal lectin was studied using disc diffusion method. GVL exhibited potent antibacterial activity against bacteria such as E.coli, S.pyogenes, S. aureus, E.faecalis, B.subtilis and coagulase-negative Staphylococci (CoNS) by disc diffusion method. Also, the results were compared with the standard antibiotic Ampicillin. These findings indicate that GVL is having therapeutic applications with great importance in clinical microbiology.

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References

Boyd and Shapleigh. Specific precipitation activity of plant agglutinins. (lectins) Science 1954; 119: 419.

Weis WI, Drickamer K. Structural basis of lectin-carbohydrate recognition. Annu Rev Biochem.1996; 65:441-473.

Peumans WJ, Van Damme EJM. Lectins as plant defense proteins. Plant Physiol. 109: 1995; 347-352.

Gabius HJ. Animal lectin. J. Biochem. 1997, 243: 543–576.

Lam SK, Ng TB Lectins: production and practical applications. Appl Microbiol Biotechnol. 2011; 89:45–55.

Kumar .S, Gupta R., Kumar G, Sahoo D, Ramesh Chander Kuhad,Bioethanol production from Gracilaria verrucosa, a red alga, in a biorefinery approach,Bioresource Technology, Volume 135,2013,Pages 150-156,ISSN 0960-8524.

Hung The Dang, Hye Ja Lee, Eun Sook Yoo, Pramod B. Shinde, Yoon Mi Lee, Jongki Hong, Dong Kyoo Kim, and Jee H. JungAnti-inflammatory Constituents of the Red Alga Gracilaria verrucosa and Their Synthetic Analogues. Journal of Natural Products 2008 71 (2), 232-240.

Dayuti, S. 2018 IOP Conf. Ser.: Earth Environ. Sci. 137 012074.

Woo, Mi-Seon & Choi, Hyeon-Son & Lee, ok-hwan & Lee, Boo-Yong. (2013). The Edible red Alga, Gracilaria verrucosa , Inhibits Lipid Accumulation and ROS Production, but Improves Glucose Uptake in 3T3-L1 Cells. Phytotherapy research : PTR. 27. 10.1002/ptr.4813.

Ogawa, Tomohisa & Watanabe, Mizuki & Naganuma, Takako & Muramoto, Koji. (2011). Diversified Carbohydrate-Binding Lectins from Marine Resources. Journal of amino acids. 2011. 838914. 10.4061/2011/838914.

Suttisrisung, Sukanya & Senapin, Saengchan & Withyachumnarnkul, Boonsirm & Wongprasert, Kanokpan. (2011). Identification and characterization of a novel legume-like lectin cDNA sequence from the red marine algae Gracilaria fisheri. Journal of biosciences. 36. 833-43. 10.1007/s12038-011-9144-8.

Singh, R. S., & Walia, A. K. (2018). Lectins from red algae and their biomedical potential. Journal of applied phycology, 30(3), 1833–1858. https://doi.org/10.1007/s10811-017-1338-5.

Benevides NMB, Holanda ML, Melo FR, Freitas ALP, Sampaio AH. Purification and partial characterization of the lectin from the marine red alga Enantiocladia duperreyi (C. Agardh) Falkenberg. Botanica Marinha. 1998;41:521–525. [Google Scholar]

Alwana A, Deignana T, O'Sullivanc M, Kellyb J, O'Farrellya C. Quantitative assay of Salmonella adherence to intestinal epithelial cells, A new method for assessing novel intervention products. Journal of Microbiological Methods. 1998;33:163–170. [Google Scholar]

Olafsen, J.P., Fletcher, T.C. an d Grand, P.J. 1992. Agglutinin activity in pacific oyster (Crassostrea gigas) haemolymph fol lowing in viro Vibrio anguillarus. Dev. Comp.Immuol.,16 (3):16.

Kanoh H, Kitamura T, Kobayashi Y. A sulfated proteoglycan from the red alga Gracilaria verrucosa is a hemagglutinin. Comparative Biochemistry and physiology. B, Comparative Biochemistry. 1992 Jul; 102(3):445-449. DOI: 10.1016/0305-0491(92)90034-o. PMID: 1499284.

Vlodavsky I., and Sachs I. Lectin receptors on the cell surface membrane and the kinetics of lectin-induced cell agglutination. Exp. Cell res. 1975; 93(1):111-9.

Doyle RJ., Keller K. Lectins in diagnostic microbiology. Eur. J. Clin. Microbiol.1984;3: 4-9.

Ravirajan, Mangaiyarkarasi & Gnanamurthy, Seenuvasan. (2014). Screening of Indian marine macro algae (Seaweeds) for haemagglutinin activity. International Journal of Advanced Research. 2. 43-52.

Liao, W.R., Lin, J., Shieh, W.Y., Jeng, W.L., & Huang, R.S. (2003). Antibiotic activity of lectins from marine algae against marine vibrios. Journal of Industrial Microbiology and Biotechnology, 30, 433-439.

Vasconcelos, M. A., Arruda, F. V., Carneiro, V. A., Silva, H. C., Nascimento, K. S., Sampaio, A. H., Cavada, B., Teixeira, E. H., Henriques, M., & Pereira, M. O. (2014). Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts. BioMed research international, 2014, 365272. https://doi.org/10.1155/2014/365272.

Sharon N., Lis H. Lectins as cell recognition molecules. Science. 1989; 246:227–234.

Sharon N and Lis H. Lectins 2nd ed. Kluwer Scientific Publishers, Amsterdam (2003).

Zem G.C., Badali O., Gaytan M., Hekmatjou H., Alvarez M., Nnoli J. Microbead analysis of cell binding to immobilized lectin: an alternative to microarrays in the development of carbohydrate drugs and diagnostic tests. Acta Histochem. 2006; 108:311–317.

Hyun S., Kim J., Kwon M., and Yu J. Selection and syntheses of tentacle type peptides as ‘artificial’ lectins against various cell-surface carbohydrates. Bioorg Med Chem. 2007; 15: 511–517.

Oppenheimer SB., Alvarez M., Nnoli J. Carbohydrate-based experimental therapeutics for cancer, HIV/AIDS and other diseases. Acta Histochem. 2008; 110:6–13.

Magalhaes A., Gomes J., Ismail MN., Haslam SM., Mendes N., Osorio H. Fut2-null mice display an altered glycosylationprofile and impaired BabA-mediated Helicobacter pylori adhesion to gastric mucosa. Glycobiology. 2009; 19:1525–36.

Mukhopadhyay B., Martins M.B., Karamanska R., Russell DA., Field RA. Bacterial detection using carbohydrate-functionalised CdS quantum dots: a model study exploiting E. coli recognition of mannosides. Tetrahedron Lett. 2009; 50:886–889.

Hamid Rabia, Akbar Masood, Ishfak H. Wani, and Shaista Rafiq., Lectins: Proteins with Diverse Applications. J App Pharm Sci. 2013; 3 (4 Suppl 1): S93-S103.

Caldeon A., Buck M. & Doyle R.J. 1997. Lectin-microorganism complexes. Electronic Lectin Journal: Lectins, Biology, Biochemistry, Clinical Biochemistry 12: 87-984583-0-2.

Hirmo S., Utt M. & Wadstrom T. 1997. Sialylglycoconjugate and proteoglycan-binding microbial lectins. Electronic Lectin Journal: Lectins, Biology, Biochemistry, Clinical Biochemistry 12: 87-984583-0-2.

Takano T, Sha Z, Peatman E, Terhune J, Liu H, Kucuktas H, Li P, Edholm ES, Wilson M, Liu Z. The two channel catfish intelectin genes exhibit highly differential patterns of tissue expression and regulation after infection with Edwardsiella ictaluri. Dev Comp Immunol. 2008;32(6):693–705. [PubMed] [Google Scholar]

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Published

2022-09-01

How to Cite

SADANANDAN , R. .; APPU, A. .; RAUF, A. A. . ANTIBACTERIAL ACTIVITY OF GVL, A LECTIN ISOLATED FROM MARINE ALGAE GRACILARIA VERRUCOSA. Journal of Fundamental and Applied Sciences, [S. l.], v. 14, n. 3, p. 509–517, 2022. DOI: 10.4314/jfas.1224. Disponível em: https://jfas.info/index.php/JFAS/article/view/1224. Acesso em: 25 sep. 2022.

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